User contributions for Xie guangneng
A user with 595 edits. Account created on 11 April 2025.
18 October 2025
- 07:4407:44, 18 October 2025 diff hist 0 XIE:2025/Oct →一、溶液的配量
- 07:4407:44, 18 October 2025 diff hist 0 XIE:2025/Oct →二、GDH 与 DPRS 的偶联
- 07:4407:44, 18 October 2025 diff hist 0 XIE:2025/Oct →一、溶液的配量
- 07:4407:44, 18 October 2025 diff hist 0 XIE:2025/Oct →一、溶液的配量
- 07:4207:42, 18 October 2025 diff hist +1 XIE:2025/Oct →electro-phoresis
- 07:4107:41, 18 October 2025 diff hist +6 XIE:2025/Oct →Conjugation
- 07:4007:40, 18 October 2025 diff hist +6 XIE:2025/Oct →Conjugation
- 03:5303:53, 18 October 2025 diff hist +53 XIE:2025/Oct →二、GDH 与 DPRS 的偶联
- 03:4903:49, 18 October 2025 diff hist 0 XIE:2025/Oct →Protocol (版本 1.1)
- 03:4803:48, 18 October 2025 diff hist −2 XIE:2025/Oct →Protocol (版本 1.1)
- 03:4603:46, 18 October 2025 diff hist −1 XIE:2025/Oct →一、溶液的配量
- 03:4103:41, 18 October 2025 diff hist 0 XIE:2025/Oct →Protocol (版本 1.1)
- 03:3803:38, 18 October 2025 diff hist 0 XIE:2025/Oct →Protocol (版本 1.1)
- 03:3403:34, 18 October 2025 diff hist +5 XIE:2025/Oct →Protocol (版本 1.1)
- 03:3203:32, 18 October 2025 diff hist +231 XIE:2025/Oct →Protocol (版本 1.1)
- 02:3602:36, 18 October 2025 diff hist +54 XIE:Project No edit summary
- 02:3502:35, 18 October 2025 diff hist +270 XIE:Project No edit summary
17 October 2025
- 12:4312:43, 17 October 2025 diff hist +17 XIE:2025/Oct No edit summary
- 12:4112:41, 17 October 2025 diff hist −1 XIE:2025/Oct No edit summary
- 12:3812:38, 17 October 2025 diff hist +60 XIE:2025/Oct No edit summary
- 12:3612:36, 17 October 2025 diff hist +114 XIE:2025/Oct No edit summary
- 12:3512:35, 17 October 2025 diff hist −1 XIE:2025/Oct No edit summary
- 12:3512:35, 17 October 2025 diff hist −210 XIE:2025/Oct No edit summary
16 October 2025
- 17:0717:07, 16 October 2025 diff hist +849 XIE:2025/Oct No edit summary
- 16:5816:58, 16 October 2025 diff hist +29 XIE:Project →Project 2: XXXXX
- 09:1809:18, 16 October 2025 diff hist +25 XIE:2025/Oct No edit summary
- 09:1609:16, 16 October 2025 diff hist +8 XIE:2025/Oct No edit summary
- 09:1509:15, 16 October 2025 diff hist +21 XIE:2025/Oct No edit summary
- 09:1109:11, 16 October 2025 diff hist +114 XIE:2025/Oct No edit summary
- 09:1009:10, 16 October 2025 diff hist +1,047 N XIE:2025/Oct Created page with "== Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === *'''''Conjugation: Proteins containing 6x Histidine at the C terminus reacted, with the chemical Bis-sulfone-DBCO for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is conjugated to the protein by click chemistry between the azide- and the DBCO-group.'''' *'''sequence(RuCHMO):CTCTCCTTCTTCCCTTTCTTT''''..."
8 October 2025
- 15:4915:49, 8 October 2025 diff hist −14 West Blot No edit summary current
- 15:4915:49, 8 October 2025 diff hist +25 West Blot No edit summary
- 15:4715:47, 8 October 2025 diff hist −3 DNA偶合物构建思路 No edit summary current Tag: Manual revert
- 15:4715:47, 8 October 2025 diff hist +3 DNA偶合物构建思路 No edit summary
- 15:4615:46, 8 October 2025 diff hist 0 DNA偶合物构建思路 No edit summary
- 15:4615:46, 8 October 2025 diff hist +21 DNA偶合物构建思路 No edit summary
- 15:3615:36, 8 October 2025 diff hist +78 DNA偶合物构建思路 No edit summary
- 15:3515:35, 8 October 2025 diff hist 0 N File:锌指蛋白 DNA.png No edit summary current
- 15:3015:30, 8 October 2025 diff hist +62 DNA偶合物构建思路 No edit summary
- 15:2915:29, 8 October 2025 diff hist 0 N File:生物正交.png No edit summary current
- 15:2715:27, 8 October 2025 diff hist +68 DNA偶合物构建思路 No edit summary
- 15:2615:26, 8 October 2025 diff hist 0 N File:Click Chemistry.jpg No edit summary current
- 15:2515:25, 8 October 2025 diff hist +54 DNA偶合物构建思路 No edit summary
- 15:2315:23, 8 October 2025 diff hist 0 N File:GCN4 Tbg.png No edit summary current
- 15:2315:23, 8 October 2025 diff hist +513 DNA偶合物构建思路 No edit summary
- 15:1015:10, 8 October 2025 diff hist +609 N DNA偶合物构建思路 Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == protein == === gene engineering and click chemistry === *'''''Click Chemistry：通过叠氮基团和炔基(环炔)环加成生成三唑环结构''''' *'''''构建生物正交系统：<br>1.在目标蛋白末端基因(N端)插入琥珀密码子(TAG-终止密码子)<br>2.在培养系统中引入特定的tRNA(特异性识别TAG位点)与其对应的氨酰-tR..."
- 14:5414:54, 8 October 2025 diff hist +29 XIE:Project →DNA Origami
- 14:4914:49, 8 October 2025 diff hist +990 West Blot No edit summary
7 October 2025
- 07:4307:43, 7 October 2025 diff hist +5 West Blot No edit summary
- 06:4806:48, 7 October 2025 diff hist +23 West Blot No edit summary