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Combined display of all available logs of yangwa. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 15:35, 8 October 2025 Xie guangneng talk contribs created page File:锌指蛋白 DNA.png
- 15:35, 8 October 2025 Xie guangneng talk contribs uploaded File:锌指蛋白 DNA.png
- 15:29, 8 October 2025 Xie guangneng talk contribs created page File:生物正交.png
- 15:29, 8 October 2025 Xie guangneng talk contribs uploaded File:生物正交.png
- 15:26, 8 October 2025 Xie guangneng talk contribs created page File:Click Chemistry.jpg
- 15:26, 8 October 2025 Xie guangneng talk contribs uploaded File:Click Chemistry.jpg
- 15:23, 8 October 2025 Xie guangneng talk contribs created page File:GCN4 Tbg.png
- 15:23, 8 October 2025 Xie guangneng talk contribs uploaded File:GCN4 Tbg.png
- 15:10, 8 October 2025 Xie guangneng talk contribs created page DNA偶合物构建思路 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == protein == === gene engineering and click chemistry === *'''''Click Chemistry：通过叠氮基团和炔基(环炔)环加成生成三唑环结构''''' *'''''构建生物正交系统：<br>1.在目标蛋白末端基因(N端)插入琥珀密码子(TAG-终止密码子)<br>2.在培养系统中引入特定的tRNA(特异性识别TAG位点)与其对应的氨酰-tR...")
- 02:57, 7 October 2025 Xie guangneng talk contribs created page Protein extraction (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 细胞总蛋白提取 == *'''配液：细胞裂解液=PIRP:PMSF=100:1, Loading buffer 蛋白上样缓冲液(5×), β-巯基乙醇(保护巯基)''' *'''提取：")
- 09:03, 6 October 2025 Hu yang talk contribs created page HU:PROJECT1/2025/Oct (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> == Method optimization for preparation of dsDNA== <ol> <li>Stock solution：dissolve aptamer to 100 μM by UP water <li>preparation of dsDNA (n=2): add 1 μL CD3e aptamer +1 μL PDGF aptamer +8 μL UP water (10 uM). Method optimization: #preheat and anneal: 95℃ 2min；70℃ 2min；60℃ 10min；50℃ 10min；40℃ 10min；37℃ 10min；RT 10min；4℃ for strorage # 37℃...")
- 08:22, 6 October 2025 Xie guangneng talk contribs created page West Blot (Created page with "蛋白提取及WB")
- 08:15, 6 October 2025 Xie guangneng talk contribs created page File:AT-DNA.png
- 08:15, 6 October 2025 Xie guangneng talk contribs uploaded File:AT-DNA.png
- 08:11, 6 October 2025 Xie guangneng talk contribs created page File:DNAforge.png
- 08:11, 6 October 2025 Xie guangneng talk contribs uploaded File:DNAforge.png
- 08:10, 6 October 2025 Xie guangneng talk contribs created page Origami (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> === DNA Origami === center|thumb|300px|DNA Origami综述 ''' 一篇关于DNA Origami的综述，列出了三代Origami的设计软件，主要针对折叠、原子模拟，其中可以下载Adential插件的SAMSON，可以模拟含蛋白质、药物分子 的DNA折纸''' === DNA forge === center|thumb|300px|DNA Origami综述 '...")
- 08:05, 6 October 2025 Xie guangneng talk contribs created page File:DNA Origami.png
- 08:05, 6 October 2025 Xie guangneng talk contribs uploaded File:DNA Origami.png
- 08:02, 6 October 2025 Xie guangneng talk contribs created page Engineered Microbes (Created page with "=== Engineerd Microbes and optoelectronic capsules === center|thumb|300px|光电微生物 ''' 讲述了构架光电微生物 EcN-NO-Lux 和 EcN-Opto-NbTNF，用于探究肠道炎症标志物硝酸盐，并光激活促进NbTNF产生释放'''")
- 07:31, 6 October 2025 Xu anran talk contribs created page XU:2025/Oct (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <html> <head> <meta charset="UTF-8"> <title>文章页面</title> </head> <body> <div class="article"> <div class="date">2025年10月5日</div> <h1 class="title">制作组会ppt</h1> <div class="content"> <p></p> <p></p> </div> </div> </body> </html>")
- 06:52, 6 October 2025 Xie guangneng talk contribs created page File:Engineered Microbes and optoelectronic capsules.png
- 06:52, 6 October 2025 Xie guangneng talk contribs uploaded File:Engineered Microbes and optoelectronic capsules.png
- 08:10, 28 September 2025 Hu yang talk contribs created page HU:Protocols/Delivery of nucleic acid (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==核酸脂质纳米粒LNP== Lipid Nanoparticle #核酸与普通化学药物的一个明显区别是核酸带有数量庞大的磷酸根，因而呈负电。为了使其能够被脂质纳米粒更好地包裹，使用一类特殊的脂质——阳离子脂质。阳离子脂质往往具有一个带铵根的亲水端，在酸性环境下能够与氢离子结合呈正电(NH4+)...")
- 01:33, 27 September 2025 Hu yang talk contribs created page HU:Protocols/He's lab management (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> 〈ol〉 〈/ol〉")
- 08:08, 26 September 2025 Hu yang talk contribs created page File:G-quadruplex-structure.png
- 08:08, 26 September 2025 Hu yang talk contribs uploaded File:G-quadruplex-structure.png
- 02:45, 24 September 2025 Hu yang talk contribs created page HU:Protocols/Design (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *Scaffold lab-made from T4 *Staples better within 60-40nt: available procurement, avoidance of structure crack")
- 02:39, 24 September 2025 Hu yang talk contribs created page HU:Project2/Q&A (Created page with "*the design of promoter from Oxford")
- 08:33, 22 September 2025 Hu yang talk contribs created page HU:常用信息/报账 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==链接== *智慧财务：微信搜索'川大智慧财务'，点击‘服务平台’，常用‘日常报销’（材料费），‘国内差旅’，‘酬金申报’ *智慧财务：四川大学财务处综合信息门户（https://fdinfo.scu.edu.cn/WFManager/login.jsp），点击‘智能报销平台’ *网上预约：四川大学财务处综合信息门户（https://f...")
- 02:36, 20 September 2025 Hu yang talk contribs moved page AAAA to HU:11333
- 02:29, 20 September 2025 Hu yang talk contribs moved page Q&A to HU:Project1/Q&A
- 02:29, 20 September 2025 Hu yang talk contribs moved page Overview to HU:Project1/Overview
- 02:19, 20 September 2025 Hu yang talk contribs moved page 其它 to HU:Protocols/其它
- 02:17, 20 September 2025 Hu yang talk contribs moved page HU:Protocols/PCR to HU:Protocols/质粒
- 02:16, 20 September 2025 Hu yang talk contribs moved page PCR to HU:Protocols/PCR
- 02:16, 20 September 2025 Hu yang talk contribs moved page 纳米粒 to HU:Protocols/纳米粒
- 02:16, 20 September 2025 Hu yang talk contribs moved page Exonuclease I to HU:Protocols/Exonuclease I
- 02:16, 20 September 2025 Hu yang talk contribs moved page PCR扩增 to HU:Protocols/PCR扩增
- 02:15, 20 September 2025 Hu yang talk contribs moved page Native Page to HU:Protocols/Native Page
- 02:15, 20 September 2025 Hu yang talk contribs moved page 琼脂糖凝胶电泳 to HU:Protocols/琼脂糖凝胶电泳
- 02:15, 20 September 2025 Hu yang talk contribs moved page OligoTaC to HU:Protocols/OligoTaC
- 02:15, 20 September 2025 Hu yang talk contribs moved page Ligation to HU:Protocols/Ligation
- 02:13, 20 September 2025 Hu yang talk contribs moved page CCK8 to HU:Protocols/CCK8
- 02:12, 20 September 2025 Hu yang talk contribs moved page 细胞房注意事项 to HU:Protocols/细胞房注意事项
- 02:12, 20 September 2025 Hu yang talk contribs moved page 常用细胞实验操作 to HU:Protocols/常用细胞实验操作
- 02:12, 20 September 2025 Hu yang talk contribs moved page 蛋白提取及WB to HU:Protocols/蛋白提取及WB
- 02:11, 20 September 2025 Hu yang talk contribs moved page 细胞提取及分选 to HU:Protocols/细胞提取及分选
- 02:11, 20 September 2025 Hu yang talk contribs moved page 流式 to HU:Protocols/流式
- 02:10, 20 September 2025 Hu yang talk contribs moved page 长期 to HU:常用信息/长期
