All public logs
Combined display of all available logs of yangwa. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 02:12, 8 December 2025 Xie guangneng talk contribs created page TEM (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Sample Preparation == *Thaw 400 uL of 2% Uranyl-formate (aliqouted in brown tube), then add 8.0 ul of 1M NaOH to the wall of tube and immediately vortex. Centrifuge for 5 min at 16000 rcf, when use, take supernatant to avoid any precipitant. ===Prepare grids=== *Put grids on parafilm wrapped glass-slides Glow-discharge them for 30sec with Plasma Cleaner (t...")
- 06:15, 7 December 2025 Chen dong talk contribs created page File:CHEN concentration screening.png
- 06:15, 7 December 2025 Chen dong talk contribs uploaded File:CHEN concentration screening.png
- 03:39, 7 December 2025 Xu anran talk contribs created page File:Nativepage 12.5(1).png
- 03:39, 7 December 2025 Xu anran talk contribs uploaded File:Nativepage 12.5(1).png
- 03:38, 7 December 2025 Xu anran talk contribs created page File:25.12.5(1).jpg
- 03:38, 7 December 2025 Xu anran talk contribs uploaded File:25.12.5(1).jpg
- 05:08, 6 December 2025 Xu anran talk contribs created page File:Nativepage 12.5.png
- 05:08, 6 December 2025 Xu anran talk contribs uploaded File:Nativepage 12.5.png
- 04:52, 6 December 2025 Xu anran talk contribs created page XU1:2025/Dec (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <html> <head> <meta charset="UTF-8"> <title>组会汇报</title> </head> <body> <div class="article"> <div class="date">2025年12月3日</div> <h1 class="title">制备TNF-SA-DNA</h1> <div class="content"> <p>1.样品稀释：生物素化TNF-α储存液 (63.4KDa 1µM)：20 µg粉末溶于317 µLdd水，分装出5管30ul冻存</p...")
- 12:31, 4 December 2025 Hu yang talk contribs created page HU:PROJECT1/2025/Dec (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==2nd Selection and Optimization of CD3-ATP Dual-aptamer system== 410px|thumb|center|{{#tag:div|ATP-CD3_Aptamer结合长度筛选|style="color: black;"}} 410px|thumb|center|{{#tag:div|ATP-CD3_Aptamer各序列结构比对|style="color: black;"}} *新增的ATP-L-611开始 结...")
- 11:55, 4 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-Aptamer结构比对-2.png
- 11:55, 4 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-Aptamer结构比对-2.png
- 11:52, 4 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-Aptamer结合长度筛选-2.png
- 11:52, 4 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-Aptamer结合长度筛选-2.png
- 07:31, 4 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-20251202 FC.PNG
- 07:31, 4 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-20251202 FC.PNG
- 07:30, 4 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-20251201 Native Page.PNG
- 07:30, 4 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-20251201 Native Page.PNG
- 02:48, 4 December 2025 Hu yang talk contribs created page HU:Protocols/细胞培养 (Created page with "===Jurkat cell culture=== #Origin： Chen Dong #Cell resuscitation: two preservation tubes in one T25 bottle（the growth of Jurkat cells is dependent on the density） #condition：suspension culture with 1640 +++ or 1640 ++-（for administering），5 ml system in T25 Cell culture flask， #medium alteration: 24h, remove 1~2mL medium and add 1~2ml fresh 1640(half alteration) #Cell passage：1/2 flask, about 2~3days once(half alteration). About 5 days,whole alteration:...")
- 13:44, 3 December 2025 Chen dong talk contribs created page File:CHEN-project1-oligoTAC-OA验证.png
- 13:44, 3 December 2025 Chen dong talk contribs uploaded File:CHEN-project1-oligoTAC-OA验证.png
- 02:51, 3 December 2025 Chen dong talk contribs created page CHEN:PROJECT1/2025/Dec (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ===Verification of DNA-ligand conjugation via click chemistry=== <ol> <li>DNA:ligand=1:2（molar ratio） <li>incubating conditions： 37 ℃ for 4 h <li>15% urea page <table border="1" cellspacing="0" cellpadding="4"> <tr> <th>Component</th> <th>Quality and Volume</th> </tr> <tr> <td>Urea</td> <td>4.2 g</td> </tr> <tr> <td>30...")
- 14:27, 2 December 2025 Chen dong talk contribs created page File:CHEN-project1-DNA-ligand conjugation.png
- 14:27, 2 December 2025 Chen dong talk contribs uploaded File:CHEN-project1-DNA-ligand conjugation.png
- 13:46, 2 December 2025 Chen dong talk contribs created page CHEN:PROJECT1/2025/Nov (Created page with "===verification of DNA-ligand conjugation via click chemistry=== #15% urea page #sample loading volume： DNA-100μM-2μL+3μL deionized water + 1 μL DNA loading buffer DNA-ligand-50μM-4μL+6μL deionized water + 2 μL DNA loading buffer #key points of protocol ##Dissolve materis in water at 37℃ except 10% APS and TEMED ##samples mixed with DNA loading buffer were heated at 95 ℃ for 5 min and immediately cooled on ice for 2 min")
- 12:30, 1 December 2025 Hu yang talk contribs moved page HU:Aabbcc to HU:Aabbccdd
- 12:30, 1 December 2025 Hu yang talk contribs created page HU:Aabbcc (Created page with "sjhdbvkjfvhlkfv")
- 12:11, 1 December 2025 Chen dong talk contribs created page CHEN:2025/Nov (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;">")
- 07:54, 1 December 2025 Hu yang talk contribs created page File:Hu-Project1-20251130 Agarose gel.png
- 07:54, 1 December 2025 Hu yang talk contribs uploaded File:Hu-Project1-20251130 Agarose gel.png
- 07:53, 1 December 2025 Hu yang talk contribs created page File:Hu-Project1-20251129 Agarose gel.png
- 07:53, 1 December 2025 Hu yang talk contribs uploaded File:Hu-Project1-20251129 Agarose gel.png
- 07:51, 1 December 2025 Xie guangneng talk contribs created page File:Oligo.xlsx
- 07:51, 1 December 2025 Xie guangneng talk contribs uploaded File:Oligo.xlsx
- 07:49, 1 December 2025 Xie guangneng talk contribs created page File:YangLab HR staples.xlsx
- 07:49, 1 December 2025 Xie guangneng talk contribs uploaded File:YangLab HR staples.xlsx
- 07:48, 1 December 2025 Xie guangneng talk contribs created page Hexagonal Prism DNA Origami (Created page with "== Material == === Scaffold === #'''P8064''' '''Powder and Stock Solution at 150 nM''' === Staples === *'''Stock solutions 1 (Hexagonal-prism DNA origami) in three 96‑well plates at 100 uM''' #'''Schematic Diagram''' thumb|center|400px|Schematic Diagram For detailed information, please refer to the shared "Origami" file on Feishu *'''Stock solutions 2 (Unmodified hexagonal‑prism DNA origami) in EP tube at 500 nM'''")
- 07:44, 1 December 2025 Xie guangneng talk contribs created page Yangwa:SharedResources (Created page with "aaaaa")
- 01:05, 1 December 2025 Xu anran talk contribs created page XU:2025/Dec (Created page with "<html> <head> <meta charset="UTF-8"> <title>文章页面</title> </head> <body> <div class="article"> <div class="date">2025年12月1日</div> <h1 class="title">组会汇报</h1> <div class="content"> <p></p> <p></p> </div> </div> </body> </html>")
- 12:33, 30 November 2025 Xie guangneng talk contribs created page File:25 11 27.png
- 12:33, 30 November 2025 Xie guangneng talk contribs uploaded File:25 11 27.png
- 03:18, 28 November 2025 Hu yang talk contribs created page File:HU-PROJECT1-CD3-ATP aptamer缩写.png
- 03:18, 28 November 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-CD3-ATP aptamer缩写.png
- 08:19, 27 November 2025 Xie guangneng talk contribs created page File:25 11 26 2.png
- 08:19, 27 November 2025 Xie guangneng talk contribs uploaded File:25 11 26 2.png
- 08:15, 27 November 2025 Xie guangneng talk contribs created page File:25 11 26 1.png
- 08:15, 27 November 2025 Xie guangneng talk contribs uploaded File:25 11 26 1.png
- 13:23, 25 November 2025 Hu yang talk contribs created page HU:Protocols/细胞周期 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==原理== 碘化丙啶(Propidium，简称PI)是一种双链DNA的荧光染料。碘化丙啶和双链DNA结合后可以产生荧光，并且荧光强度和双链DNA的含量成正比。细胞内的DNA被碘化丙啶染色后，可以用流式细胞仪对细胞进行DNA含量测定，然后根据DNA含量的分布情况，可以进行细胞周期和细胞凋亡分析。 碘...")
- 13:21, 25 November 2025 Xie guangneng talk contribs created page File:Staples.png
.png){kind=link}
.jpg){kind=link}
