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13:21, 25 November 2025 Xie guangneng talk contribs created page File:Staples.png
13:21, 25 November 2025 Xie guangneng talk contribs uploaded File:Staples.png
13:19, 25 November 2025 Xie guangneng talk contribs created page Construction of DNA-Origami (Created page with "== Construction Material == === Scaffold === #'''P8064''' '''Powder and Stock Solution at 200 nM''' === Staples === #'''Stock solutions in three 96‑well plates at 100 nM''' *'''Schematic Diagram''' File:Staples|thuemb|")
10:09, 17 November 2025 Xie guangneng talk contribs created page XIE:2025/Dec (Created page with "XIE:Project 1: Protein Conjugation to DNA Origami/protocol")
10:32, 14 November 2025 Xie guangneng talk contribs created page File:RuCHMO S2 11 13.jpg
10:32, 14 November 2025 Xie guangneng talk contribs uploaded File:RuCHMO S2 11 13.jpg
10:29, 14 November 2025 Xie guangneng talk contribs created page File:RuCHMO S2 11 11.jpg
10:29, 14 November 2025 Xie guangneng talk contribs uploaded File:RuCHMO S2 11 11.jpg
12:04, 6 November 2025 Xie guangneng talk contribs uploaded a new version of File:25 11 6 GDH S1.jpg
12:02, 6 November 2025 Xie guangneng talk contribs created page File:25 11 6 GDH S1.jpg
12:02, 6 November 2025 Xie guangneng talk contribs uploaded File:25 11 6 GDH S1.jpg
02:34, 5 November 2025 Xie guangneng talk contribs created page File:Page1.jpg
02:34, 5 November 2025 Xie guangneng talk contribs uploaded File:Page1.jpg
12:24, 4 November 2025 Xie guangneng talk contribs created page File:Reference of gel concentration.jpg
12:24, 4 November 2025 Xie guangneng talk contribs uploaded File:Reference of gel concentration.jpg
08:45, 3 November 2025 Xie guangneng talk contribs created page XIE/Enzyme:2025/Nov (Created page with "== 日期 11.3 == === 1. GDH === 取 '''GDH 1.4 mg'''，溶解于 '''2 mL PBS (1×)''' 中，形成 '''20 μM''' 溶液。分装于离心管（每管 '''30 μL'''，共 '''4 管'''），以及 EP 管（'''400 μL、600 μL、800 μL''' 各一管）。取其中一管，加入 '''90 mL PBS (1×, 5 μM)'''。取 '''100 μL''' 作为待反应液，剩余 '''20 μL''' 作为凝胶对照液。 === 2. DBCO === 称取 '''DBCO 5 mg'''，加入 '''50 μL DMF (100 mM)''...")
08:22, 31 October 2025 Xie guangneng talk contribs created page Protocol (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con...")
12:04, 30 October 2025 Xie guangneng talk contribs created page File:T cell.png
12:04, 30 October 2025 Xie guangneng talk contribs uploaded File:T cell.png
11:17, 30 October 2025 Xie guangneng talk contribs created page Background (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 一、传统的T细胞激活 == === 1. 抗原识别 (Signal 1) === ==== 1.1 ==== '''APC（抗原呈递细胞）'''：吞噬/摄取抗原，并向 '''T细胞呈递MHC分子'''（signal 1），并提供'''共刺激分子 B7 (signal 2)'''。 '''TCR（T细胞表面）'''：特异性识别 MHC 分子呈递的抗原肽，与 '''CD3复合体'''（CD3γ, δ,...")
11:11, 30 October 2025 Xie guangneng talk contribs created page 设计工具 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Wire frame == [https://dnaforge.org DNAforge 官方网站] === 步骤 === '''3D 图形 → 路径渲染 → 圆柱体渲染 → 核酸渲染''' === OxDNA 模拟 === * 打开 '''Docker''' * 在控制面板中输入以下命令： # ls # cd dnaforge-backend # docker compose up -d == 紧密型 == '''cadnano2''' == 步骤 == '''蜂窝/方型绘制...")
10:23, 30 October 2025 Xie guangneng talk contribs created page XIE/EnZyme:2025/Oct (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con...")
10:46, 21 October 2025 Xie guangneng talk contribs created page Information (Created page with "== 一、传统的T细胞激活 == === 1. 抗原识别 (Signal 1) === ==== 1.1 ==== '''APC（抗原呈递细胞）'''：吞噬/摄取抗原，并向 '''T细胞呈递MHC分子'''（signal 1），并提供'''共刺激分子 B7 (signal 2)'''。 '''TCR（T细胞表面）'''：特异性识别 MHC 分子呈递的抗原肽，与 '''CD3复合体'''（CD3γ, δ, ε, ζ）组成 '''TCR复合体'''。 CD3ζ上的 '''ITAM (immunoreceptor tyrosine-based activation motifs)''' 被...")
09:10, 16 October 2025 Xie guangneng talk contribs created page XIE:2025/Oct (Created page with "== Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === *'''''Conjugation: Proteins containing 6x Histidine at the C terminus reacted, with the chemical Bis-sulfone-DBCO for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is conjugated to the protein by click chemistry between the azide- and the DBCO-group.'''' *'''sequence(RuCHMO):CTCTCCTTCTTCCCTTTCTTT''''...")
15:35, 8 October 2025 Xie guangneng talk contribs created page File:锌指蛋白 DNA.png
15:35, 8 October 2025 Xie guangneng talk contribs uploaded File:锌指蛋白 DNA.png
15:29, 8 October 2025 Xie guangneng talk contribs created page File:生物正交.png
15:29, 8 October 2025 Xie guangneng talk contribs uploaded File:生物正交.png
15:26, 8 October 2025 Xie guangneng talk contribs created page File:Click Chemistry.jpg
15:26, 8 October 2025 Xie guangneng talk contribs uploaded File:Click Chemistry.jpg
15:23, 8 October 2025 Xie guangneng talk contribs created page File:GCN4 Tbg.png
15:23, 8 October 2025 Xie guangneng talk contribs uploaded File:GCN4 Tbg.png
15:10, 8 October 2025 Xie guangneng talk contribs created page DNA偶合物构建思路 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == protein == === gene engineering and click chemistry === *'''''Click Chemistry：通过叠氮基团和炔基(环炔)环加成生成三唑环结构''''' *'''''构建生物正交系统：<br>1.在目标蛋白末端基因(N端)插入琥珀密码子(TAG-终止密码子)<br>2.在培养系统中引入特定的tRNA(特异性识别TAG位点)与其对应的氨酰-tR...")
02:57, 7 October 2025 Xie guangneng talk contribs created page Protein extraction (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 细胞总蛋白提取 == *'''配液：细胞裂解液=PIRP:PMSF=100:1, Loading buffer 蛋白上样缓冲液(5×), β-巯基乙醇(保护巯基)''' *'''提取：")
08:22, 6 October 2025 Xie guangneng talk contribs created page West Blot (Created page with "蛋白提取及WB")
08:15, 6 October 2025 Xie guangneng talk contribs created page File:AT-DNA.png
08:15, 6 October 2025 Xie guangneng talk contribs uploaded File:AT-DNA.png
08:11, 6 October 2025 Xie guangneng talk contribs created page File:DNAforge.png
08:11, 6 October 2025 Xie guangneng talk contribs uploaded File:DNAforge.png
08:10, 6 October 2025 Xie guangneng talk contribs created page Origami (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> === DNA Origami === center|thumb|300px|DNA Origami综述 ''' 一篇关于DNA Origami的综述，列出了三代Origami的设计软件，主要针对折叠、原子模拟，其中可以下载Adential插件的SAMSON，可以模拟含蛋白质、药物分子的DNA折纸''' === DNA forge === center|thumb|300px|DNA Origami综述 '...")
08:05, 6 October 2025 Xie guangneng talk contribs created page File:DNA Origami.png
08:05, 6 October 2025 Xie guangneng talk contribs uploaded File:DNA Origami.png
08:02, 6 October 2025 Xie guangneng talk contribs created page Engineered Microbes (Created page with "=== Engineerd Microbes and optoelectronic capsules === center|thumb|300px|光电微生物 ''' 讲述了构架光电微生物 EcN-NO-Lux 和 EcN-Opto-NbTNF，用于探究肠道炎症标志物硝酸盐，并光激活促进NbTNF产生释放'''")
06:52, 6 October 2025 Xie guangneng talk contribs created page File:Engineered Microbes and optoelectronic capsules.png
06:52, 6 October 2025 Xie guangneng talk contribs uploaded File:Engineered Microbes and optoelectronic capsules.png
06:57, 16 September 2025 Xie guangneng talk contribs created page Wireframe (Created page with "== wireframe == <html> <details> <summary style="font-size: 20px; font-weight: bold;">📁 </summary> </html> <div style="border: 1px solid #ccc; padding: 10px; background-color: #f8f8f8;"> '''DNAforge''' </div>")
11:13, 11 September 2025 Xie guangneng talk contribs created page XIE:2025/Sep (Created page with " '''== 实验记录 ==''' '''== 文献 =='''")
11:02, 11 September 2025 Xie guangneng talk contribs created page Yangwa:Order (Created page with "=== 常用信息 === * 日程安排 # 常规事项时间安排 # 关键事项时间安排 * 注意事项 * XXX === 实验记录 === * XXX实验 # 实验数据 # XXX * XXX实验 # 实验数据 # XXX === 文献 === * XXX实验 * XXX实验")
21:50, 11 April 2025 Xie guangneng talk contribs created page XIE:Project (Created page with "=My projects= ==Project 1== ==Project 2==")
21:13, 11 April 2025 User account Xie guangneng talk contribs was created automatically