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流式测定
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From yangwa
流式测定
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在进行细胞分选前后,均移取部分细胞悬液,加入CD8+抗体,用于后续流式测定T cell的占比,考察试剂盒分选效率
在加入CFSE溶液前后,均移取部分T cell悬液,流式测定cell CFSE信号,用作cell传代的阴性和阳性对照。
72h后流式测定细胞传代
CFSE荧光强度随细胞分裂递减
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