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  • 15:10, 8 October 2025 Xie guangneng talk contribs created page DNA偶合物构建思路 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == protein == === gene engineering and click chemistry === *'''''Click Chemistry:通过叠氮基团和炔基(环炔)环加成生成三唑环结构''''' *'''''构建生物正交系统:<br>1.在目标蛋白末端基因(N端)插入琥珀密码子(TAG-终止密码子)<br>2.在培养系统中引入特定的tRNA(特异性识别TAG位点)与其对应的氨酰-tR...")