All public logs
Combined display of all available logs of yangwa. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 02:09, 20 September 2025 Hu yang talk contribs moved page 近期 to HU:常用信息/近期
- 02:07, 20 September 2025 Hu yang talk contribs moved page 账号及密码 to HU:常用信息/账号及密码
- 08:21, 19 September 2025 Hu yang talk contribs created page HU:Project1/Q&A-2 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *How do we determine the Aptamers can work？ CD3：After attaching to Jurkat T cell， the Fluorophore From aptamer can be detected by FC<br> PDGF:After attaching to PDGF protein， Agarose gel can distinguish the RNA binding to PDGF or not.(gold view) *afirm Dissociation of dsDNA afer adding PDGF #FRET #Agarose gel:Free CD3 Aptamer increase,PDGF aptamer binding to protein i...")
- 07:38, 18 September 2025 Hu yang talk contribs uploaded a new version of File:HY Head Photo.jpg
- 07:18, 16 September 2025 Xu anran talk contribs created page XU:详情 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> center|thumb|300px|")
- 07:17, 16 September 2025 Xu anran talk contribs created page 详情 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> center|thumb|300px|")
- 06:57, 16 September 2025 Xie guangneng talk contribs created page Wireframe (Created page with "== wireframe == <html> <details> <summary style="font-size: 20px; font-weight: bold;">📁 </summary> </html> <div style="border: 1px solid #ccc; padding: 10px; background-color: #f8f8f8;"> '''DNAforge''' </div>")
- 12:31, 15 September 2025 Xu anran talk contribs created page XU:Insulin DNA origami (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> center|thumb|300px")
- 12:25, 15 September 2025 Xu anran talk contribs created page XU:DNAforge (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> center|thumb|300px")
- 12:17, 15 September 2025 Xu anran talk contribs created page DNAforge (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==流式测定== #在进行细胞分选前后，均移取部分细胞悬液，加入CD8+抗体，用于后续流式测定T cell的占比，考察试剂盒分选效率 #在加入CFSE溶液前后，均移取部分T cell悬液，流式测定cell CFSE信号，用作cell传代的阴性和阳性对照。 #72h后流式测定细胞传代 File:CFSE荧光强度随细胞分裂递减...")
- 12:12, 15 September 2025 Hu yang talk contribs moved page AAAA to HU:B/AAAA
- 12:07, 15 September 2025 Hu yang talk contribs moved page AA to AAAA
- 12:06, 15 September 2025 Hu yang talk contribs created page AA (Created page with "SDSDF")
- 12:01, 15 September 2025 Hu yang talk contribs created page AADD (Created page with "SHBDJHKS")
- 11:31, 15 September 2025 Xu anran talk contribs created page File:InsulinDNAorigami.pdf
- 11:31, 15 September 2025 Xu anran talk contribs uploaded File:InsulinDNAorigami.pdf
- 09:21, 15 September 2025 Xu anran talk contribs created page File:DNAforge.pdf
- 09:21, 15 September 2025 Xu anran talk contribs uploaded File:DNAforge.pdf
- 08:49, 15 September 2025 Xu anran talk contribs created page XU:2025/Sep (Created page with "<!DOCTYPE html> <html lang="zh-CN"> <head> <meta charset="UTF-8"> <meta name="viewport" content="width=device-width, initial-scale=1.0"> <title>文档展示中心</title> <link rel="stylesheet" href="https://cdnjs.cloudflare.com/ajax/libs/font-awesome/6.4.0/css/all.min.css"> <style> * { margin: 0; padding: 0; box-sizing: border-box; font-family: 'Segoe UI', Tahoma, Geneva, Verdana, sans-serif;...")
- 11:13, 11 September 2025 Xie guangneng talk contribs created page XIE:2025/Sep (Created page with " '''== 实验记录 ==''' '''== 文献 =='''")
- 11:02, 11 September 2025 Xie guangneng talk contribs created page Yangwa:Order (Created page with "=== 常用信息 === * 日程安排 # 常规事项时间安排 # 关键事项时间安排 * 注意事项 * XXX === 实验记录 === * XXX实验 # 实验数据 # XXX * XXX实验 # 实验数据 # XXX === 文献 === * XXX实验 * XXX实验")
- 02:42, 9 September 2025 Hu yang talk contribs created page Exonuclease I (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *Enzymatic PCR Cleanup Protocol :To remove surplus primers and dNTP for further application <ol> <li>Set-up the reaction as follows: <table class="wikitable" style="margin: auto; border-collapse: collapse; border: 1px solid #a2a9b1;"> <tr> <th>COMPONENTS</th> <th>Reaction Volumes</th> </tr> <tr> <td>PCR Product</td> <td>5 μl</td>...")
- 08:54, 6 September 2025 Hu yang talk contribs created page File:TCR激活（第一信号）.jpg
- 08:54, 6 September 2025 Hu yang talk contribs uploaded File:TCR激活（第一信号）.jpg
- 08:53, 6 September 2025 Hu yang talk contribs created page File:TCR激活（第二信号）.jpg
- 08:53, 6 September 2025 Hu yang talk contribs uploaded File:TCR激活（第二信号）.jpg
- 08:53, 6 September 2025 Hu yang talk contribs created page File:TCR-CD3复合物结构.jpg
- 08:53, 6 September 2025 Hu yang talk contribs uploaded File:TCR-CD3复合物结构.jpg
- 13:31, 5 September 2025 Hu yang talk contribs created page Q&A (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <ol> <li>Q：CD3和CD28的连接比例如何控制 </ol>")
- 13:00, 5 September 2025 Hu yang talk contribs created page File:三特异性抗体原理图2.png
- 13:00, 5 September 2025 Hu yang talk contribs uploaded File:三特异性抗体原理图2.png
- 13:00, 5 September 2025 Hu yang talk contribs created page File:三特异性抗体原理图1.png
- 13:00, 5 September 2025 Hu yang talk contribs uploaded File:三特异性抗体原理图1.png
- 12:49, 5 September 2025 Hu yang talk contribs created page Overview (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;">")
- 13:31, 4 September 2025 Hu yang talk contribs created page 其它 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> #进样小瓶清洗： 泡入乙醇，超声，搽去字迹；自来水清洗；UP水清洗")
- 11:04, 4 September 2025 Hu yang talk contribs created page PCR扩增 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ===原理=== <ol> <li><strong>PCR</strong>:通常指的是普通PCR，以双链DNA为模板，以dNTP为底物，定性扩增双链DNA。大致可分为高温变性、低温退火、适温延伸三个基本反应步骤。 <li><strong>qPCR或Real-time PCR</strong>:指的是实时荧光定量PCR，以cDNA为模板，以dNTP 为底物，对扩增出的DNA进行<strong>相对定...")
- 10:42, 4 September 2025 Hu yang talk contribs created page 长期 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> #DNA Origami综述,DDL:12月 #专利")
- 10:39, 4 September 2025 Hu yang talk contribs created page 近期 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> 20250904 组会 *陈栋师兄课题 #多价Oligotac 21bp循环多价构建（另一侧是否可以保留41nt），价态3 6 #环状126bp，Oligotac单体同时用作splint（10+11） #PCR：购买试剂，包括PCR试剂盒（NEB luna PCR）、DNA外切酶 *三抗 #购买试剂")
- 14:03, 3 September 2025 Hu yang talk contribs created page Native Page (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> 18孔梳，上样量10pmol（1 uM,10uL）")
- 13:45, 3 September 2025 Hu yang talk contribs created page 账号及密码 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==房间密码== *电梯：1234 *PCR，荧光：1348 *WB，酶标板：248965 ==其它== *何组公共实验平台密码：2589")
- 13:08, 3 September 2025 Hu yang talk contribs created page HU:2025/Sep (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *第一周 #细胞铺板 Oligotac制备 脂质体制备 #细胞给药（Oligotac不同价态 脂质体浓度） #WB 流式（脂质体MCHI表达）")
- 09:09, 19 August 2025 Hu yang talk contribs moved page 2222 to 1111
- 09:08, 19 August 2025 Hu yang talk contribs created page 2222 (Created page with "Test")
- 09:36, 18 August 2025 Hu yang talk contribs created page OligoTaC (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==OligoTaC制备== <ol> <li>单链DNA：100 uM in H2O <li>单价Oligotac制备 <ol> <li>制备一价双链DNA：分别取5 ul互补DNA单链，加入10 ul UP水 <li>37度摇床1.5 h <li>RT overnight </ol> <li>多价Oligotac制备 <ol>??? <li>取5ul上诉单价体系，加入多价单链DNA 5 ul <li>37度摇床1.5 h <li>琼脂糖凝胶验证")
- 14:47, 12 August 2025 Hu yang talk contribs created page Ligation (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <ol> <li><strong>Ligation Protocol with T4 DNA Ligase (M0202)</strong></br> <ol> <li>Set up the following reaction in a microcentrifuge tube on ice. ( Note that the table shows a ligation using a <strong>molar ratio</strong> of 1:3 vector to insert for the indicated DNA sizes.) Use NEBioCalculator to calculate molar ratios. <table class="wikitable" style="margin: 0 auto; bo...")
- 13:56, 12 August 2025 Hu yang talk contribs moved page 常用细胞房操作 to HU:1
- 13:53, 12 August 2025 Hu yang talk contribs created page 常用细胞实验操作 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==更换培养基== #更换频率1次/d。 #弃去旧培养基： ##贴壁细胞（如癌细胞）：移液枪直接吸取培养基并弃去； ##悬浮细胞：收集原培养基及cell置于EP管中，离心，弃上清： ##半贴壁细胞：采用半换液，倾斜孔板或培养皿，移液枪吸取上层培养基弃去。 #无需PBS清洗,直接加入新鲜培养基。...")
- 13:40, 12 August 2025 Hu yang talk contribs created page 纳米粒 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <ol> <li> <strong>脂质体</strong> <ol> <li> <strong>胆固醇</strong> <p>胆固醇属于两亲物质，同时具有亲油和亲水两种基团，但是其亲油性较亲水性强，与磷脂相互作用形成脂质体时可嵌入到磷脂分子之间，调节膜结构及性质...")
- 14:21, 11 August 2025 Hu yang talk contribs created page HU:2025/Aug (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *第三周 #cell铺板，脂质体制备，oligo体系合成")
- 06:51, 26 July 2025 Hu yang talk contribs created page 流式 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <big>'''一、操作'''</big> #CD8+ T cell单染PE-H(A) #人源性CD8 T MHCI-I染色：FBS溶液封闭+鼠抗人MHCI+山羊抗鼠荧光抗体 #进样前培养基更换为PBS，避免污染培养基。吸取染色后的cell，PBS稀释，Cell总量约为e6-e5，转移至流式管中500ul样本上样（每次进样仅需约30 ul） #阴性管：只有细胞，没有荧光抗...")
