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• 01:37, 7 July 2025 Hu yang talk contribs created page 蛋白提取及WB (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==目的== *药效（药效前应当先做毒性） ==蛋白提取== #移液枪转移12孔板中cell培养液到ep管中，底部留存部分液态培养基； #胰酶或者细胞刮板分离底部粘璧cell，移液枪转移至上述ep管中。流式不可采用刮板，会损伤cell；刮板用PBS清洗； #离心2k rpm 3min，弃去上清，PBS重悬，n合1，再次离心...")