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• 02:11, 20 September 2025 Hu yang talk contribs moved page 流式 to HU:Protocols/流式
• 06:51, 26 July 2025 Hu yang talk contribs created page 流式 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <big>'''一、操作'''</big> #CD8+ T cell单染PE-H(A) #人源性CD8 T MHCI-I染色：FBS溶液封闭+鼠抗人MHCI+山羊抗鼠荧光抗体 #进样前培养基更换为PBS，避免污染培养基。吸取染色后的cell，PBS稀释，Cell总量约为e6-e5，转移至流式管中500ul样本上样（每次进样仅需约30 ul） #阴性管：只有细胞，没有荧光抗...")