DNAforge

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Revision as of 12:17, 15 September 2025 by Xu anran (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==流式测定== #在进行细胞分选前后，均移取部分细胞悬液，加入CD8+抗体，用于后续流式测定T cell的占比，考察试剂盒分选效率 #在加入CFSE溶液前后，均移取部分T cell悬液，流式测定cell CFSE信号，用作cell传代的阴性和阳性对照。 #72h后流式测定细胞传代 File:CFSE荧光强度随细胞分裂递减...")
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流式测定[edit]

  1. 在进行细胞分选前后，均移取部分细胞悬液，加入CD8+抗体，用于后续流式测定T cell的占比，考察试剂盒分选效率
  2. 在加入CFSE溶液前后，均移取部分T cell悬液，流式测定cell CFSE信号，用作cell传代的阴性和阳性对照。
  3. 72h后流式测定细胞传代
CFSE荧光强度随细胞分裂递减
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