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Combined display of all available logs of yangwa. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 07:50, 9 November 2025 Xu anran talk contribs created page File:流式1.jpg
- 07:50, 9 November 2025 Xu anran talk contribs uploaded File:流式1.jpg
- 12:20, 7 November 2025 Hu yang talk contribs uploaded a new version of File:Hu-Protocols-triple helix formation in a DNA frame.png
- 12:14, 7 November 2025 Hu yang talk contribs created page File:Hu-Protocols-triple helix formation in a DNA frame.png
- 12:14, 7 November 2025 Hu yang talk contribs uploaded File:Hu-Protocols-triple helix formation in a DNA frame.png
- 12:05, 7 November 2025 Hu yang talk contribs created page File:Hoogsteen pairing doi 10.1093-nar-gkv241 .png
- 12:05, 7 November 2025 Hu yang talk contribs uploaded File:Hoogsteen pairing doi 10.1093-nar-gkv241 .png
- 11:55, 7 November 2025 Hu yang talk contribs uploaded a new version of File:G-quadruplex-structure.png
- 08:57, 7 November 2025 Hu yang talk contribs created page File:HU-Project1-20251107dsDNA与Jurkat结合结果.png
- 08:57, 7 November 2025 Hu yang talk contribs uploaded File:HU-Project1-20251107dsDNA与Jurkat结合结果.png
- 02:33, 7 November 2025 Hu yang talk contribs created page HU:Protocols/细胞凋亡 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==原理== Annexin V-YSFluor"647/7-AAD细胞调亡检试剂盒是用荧光染料YSFluo 647 (Phycoerythrin)标记的Annexiny作为探针，来检测细胞早期调亡的发生，可用荧光显微镜、流式细胞仅或其他荧光检测设备进行检测。 #凋亡细胞：在正常的活细胞中，磷脂酰丝氨酸(phosphotidylserine,PS)位于细胞膜的内侧，但在...")
- 12:38, 6 November 2025 Hu yang talk contribs created page File:HU-Project1-251106dsDNA与Jurkat结合.png
- 12:38, 6 November 2025 Hu yang talk contribs uploaded File:HU-Project1-251106dsDNA与Jurkat结合.png
- 12:04, 6 November 2025 Xie guangneng talk contribs uploaded a new version of File:25 11 6 GDH S1.jpg
- 12:02, 6 November 2025 Xie guangneng talk contribs created page File:25 11 6 GDH S1.jpg
- 12:02, 6 November 2025 Xie guangneng talk contribs uploaded File:25 11 6 GDH S1.jpg
- 06:02, 5 November 2025 Hu yang talk contribs created page File:HU-Project1-2510map.xlsx
- 06:02, 5 November 2025 Hu yang talk contribs uploaded File:HU-Project1-2510map.xlsx
- 02:34, 5 November 2025 Xie guangneng talk contribs created page File:Page1.jpg
- 02:34, 5 November 2025 Xie guangneng talk contribs uploaded File:Page1.jpg
- 02:02, 5 November 2025 Hu yang talk contribs uploaded a new version of File:Hu-Project1-CD3-Jurkat cell binding（with random sequence）.jpg
- 01:59, 5 November 2025 Hu yang talk contribs created page File:Hu-Project1-CD3-Jurkat cell binding（with random sequence）.jpg
- 01:59, 5 November 2025 Hu yang talk contribs uploaded File:Hu-Project1-CD3-Jurkat cell binding（with random sequence）.jpg
- 12:24, 4 November 2025 Xie guangneng talk contribs created page File:Reference of gel concentration.jpg
- 12:24, 4 November 2025 Xie guangneng talk contribs uploaded File:Reference of gel concentration.jpg
- 08:45, 3 November 2025 Xie guangneng talk contribs created page XIE/Enzyme:2025/Nov (Created page with "== 日期 11.3 == === 1. GDH === 取 '''GDH 1.4 mg'''，溶解于 '''2 mL PBS (1×)''' 中，形成 '''20 μM''' 溶液。 分装于离心管（每管 '''30 μL'''，共 '''4 管'''），以及 EP 管（'''400 μL、600 μL、800 μL''' 各一管）。 取其中一管，加入 '''90 mL PBS (1×, 5 μM)'''。 取 '''100 μL''' 作为待反应液，剩余 '''20 μL''' 作为凝胶对照液。 === 2. DBCO === 称取 '''DBCO 5 mg'''，加入 '''50 μL DMF (100 mM)''...")
- 02:18, 3 November 2025 Xu anran talk contribs created page XU:2025/Nov (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <html> <head> <meta charset="UTF-8"> <title>组会汇报</title> </head> <body> <div class="article"> <div class="date">2025年11月3日</div> <h1 class="title">组会汇报</h1> <div class="content"> <p>有人坐下来，和谢广能一起做</p> <p></p> </div> </div> </body> </html>")
- 08:22, 31 October 2025 Xie guangneng talk contribs created page Protocol (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con...")
- 03:42, 31 October 2025 Xu anran talk contribs created page File:图片1.png
- 03:42, 31 October 2025 Xu anran talk contribs uploaded File:图片1.png
- 02:02, 31 October 2025 Hu yang talk contribs created page HU:PROJECT1/2025/Nov (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==Reliability of ATP Aptamer== *Formation of signal dsDNA and CD3+ATP dsDNA（after incubation, diluete to 25nM） #37℃ 2h #Groups: ATP Aptamer; Signal DNA;CD3 aptamer;fresh×2; dsDNA×2 *Addition of ATP: ATP dsDNA at 40 nM was incubated in the absence of ATP for 10 min at 37 ℃, followed by the addition of ATP to 1 mM and a further incubation at the same temperature for...")
- 12:04, 30 October 2025 Xie guangneng talk contribs created page File:T cell.png
- 12:04, 30 October 2025 Xie guangneng talk contribs uploaded File:T cell.png
- 11:17, 30 October 2025 Xie guangneng talk contribs created page Background (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 一、传统的T细胞激活 == === 1. 抗原识别 (Signal 1) === ==== 1.1 ==== '''APC（抗原呈递细胞）'''：吞噬/摄取抗原，并向 '''T细胞呈递MHC分子'''（signal 1），并提供'''共刺激分子 B7 (signal 2)'''。 '''TCR（T细胞表面）'''：特异性识别 MHC 分子呈递的抗原肽，与 '''CD3复合体'''（CD3γ, δ,...")
- 11:11, 30 October 2025 Xie guangneng talk contribs created page 设计工具 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Wire frame == [https://dnaforge.org DNAforge 官方网站] === 步骤 === '''3D 图形 → 路径渲染 → 圆柱体渲染 → 核酸渲染''' === OxDNA 模拟 === * 打开 '''Docker''' * 在控制面板中输入以下命令： # ls # cd dnaforge-backend # docker compose up -d == 紧密型 == '''cadnano2''' == 步骤 == '''蜂窝/方型绘制...")
- 10:23, 30 October 2025 Xie guangneng talk contribs created page XIE/EnZyme:2025/Oct (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con...")
- 06:33, 29 October 2025 Hu yang talk contribs created page File:HY-20251029-Binding of the cd3 against Jurkat-2.png
- 06:33, 29 October 2025 Hu yang talk contribs uploaded File:HY-20251029-Binding of the cd3 against Jurkat-2.png
- 06:32, 29 October 2025 Hu yang talk contribs created page File:HY-20251029-Binding of the cd3 against Jurkat.png
- 06:32, 29 October 2025 Hu yang talk contribs uploaded File:HY-20251029-Binding of the cd3 against Jurkat.png
- 03:16, 29 October 2025 Hu yang talk contribs uploaded a new version of File:HY Head Photo.jpg
- 10:46, 21 October 2025 Xie guangneng talk contribs created page Information (Created page with "== 一、传统的T细胞激活 == === 1. 抗原识别 (Signal 1) === ==== 1.1 ==== '''APC（抗原呈递细胞）'''：吞噬/摄取抗原，并向 '''T细胞呈递MHC分子'''（signal 1），并提供'''共刺激分子 B7 (signal 2)'''。 '''TCR（T细胞表面）'''：特异性识别 MHC 分子呈递的抗原肽，与 '''CD3复合体'''（CD3γ, δ, ε, ζ）组成 '''TCR复合体'''。 CD3ζ上的 '''ITAM (immunoreceptor tyrosine-based activation motifs)''' 被...")
- 10:53, 20 October 2025 Hu yang talk contribs created page File:HY 2510单蛋白孵育及对dsDNA形成的影响.png
- 10:53, 20 October 2025 Hu yang talk contribs uploaded File:HY 2510单蛋白孵育及对dsDNA形成的影响.png
- 14:42, 18 October 2025 Xu anran talk contribs created page File:文献.png
- 14:42, 18 October 2025 Xu anran talk contribs uploaded File:文献.png
- 14:39, 18 October 2025 Xu anran talk contribs created page File:9f6d042d-367e-42b4-9f23-1b9ab1df5cb0.png
- 14:39, 18 October 2025 Xu anran talk contribs uploaded File:9f6d042d-367e-42b4-9f23-1b9ab1df5cb0.png
- 09:10, 16 October 2025 Xie guangneng talk contribs created page XIE:2025/Oct (Created page with "== Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === *'''''Conjugation: Proteins containing 6x Histidine at the C terminus reacted, with the chemical Bis-sulfone-DBCO for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is conjugated to the protein by click chemistry between the azide- and the DBCO-group.'''' *'''sequence(RuCHMO):CTCTCCTTCTTCCCTTTCTTT''''...")
- 06:02, 14 October 2025 Hu yang talk contribs created page HU:常用信息/课程 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==药代动== #三次讨论")
