User contributions for Xie guangneng
A user with 501 edits. Account created on 11 April 2025.
4 November 2025
- 08:5008:50, 4 November 2025 diff hist +34 XIE/Enzyme:2025/Nov →日期 11.4
- 08:4908:49, 4 November 2025 diff hist +13 XIE/Enzyme:2025/Nov →日期 11.4
- 08:4808:48, 4 November 2025 diff hist +244 XIE/Enzyme:2025/Nov →日期 11.3
- 08:4208:42, 4 November 2025 diff hist +11 Protocol →1. 制胶
- 08:4108:41, 4 November 2025 diff hist +2 Protocol →== 二、Nativepage
- 08:4108:41, 4 November 2025 diff hist +31 Protocol →Nativepage
- 08:4008:40, 4 November 2025 diff hist +2 Protocol →制胶
- 08:4008:40, 4 November 2025 diff hist +6 Protocol →缓冲液配置
- 08:3908:39, 4 November 2025 diff hist +6 Protocol →缓冲液配置
- 08:3908:39, 4 November 2025 diff hist −5 Protocol →10X TBE (1L)
- 08:3908:39, 4 November 2025 diff hist +2 Protocol →缓冲液配置
- 08:3808:38, 4 November 2025 diff hist −6 Protocol →Nativepage
- 08:3808:38, 4 November 2025 diff hist +316 Protocol →8. 凝胶电泳验证杂交情况
3 November 2025
- 11:3911:39, 3 November 2025 diff hist +1 Protocol →一、酶核酸杂交体的制备
- 11:3811:38, 3 November 2025 diff hist −1 Protocol →Protocol (版本 1.0)
- 11:3811:38, 3 November 2025 diff hist +6 Protocol →Protocol (版本 1.0)
- 11:3711:37, 3 November 2025 diff hist −4 Protocol →Protocol (版本 1.0)
- 11:3711:37, 3 November 2025 diff hist +5 Protocol →一、酶核酸杂交体的制备
- 11:3711:37, 3 November 2025 diff hist +6 Protocol →一、酶核酸杂交体的制备
- 11:3611:36, 3 November 2025 diff hist −30 Protocol →Protocol (版本 1.0)
- 11:3611:36, 3 November 2025 diff hist 0 Protocol →Protocol (版本 1.0)
- 11:3511:35, 3 November 2025 diff hist −2 Protocol →Protocol (版本 1.0)
- 11:3511:35, 3 November 2025 diff hist +146 Protocol →Protocol (版本 1.0)
- 11:3011:30, 3 November 2025 diff hist 0 Protocol →Protocol (版本 1.0)
- 11:3011:30, 3 November 2025 diff hist +48 Protocol →Protocol (版本 1.0)
- 09:4909:49, 3 November 2025 diff hist +130 XIE/Enzyme:2025/Nov →4.反应
- 09:3009:30, 3 November 2025 diff hist +6 XIE/Enzyme:2025/Nov →1. GDH
- 09:2909:29, 3 November 2025 diff hist +4 XIE/Enzyme:2025/Nov →日期 11.3
- 09:2909:29, 3 November 2025 diff hist 0 XIE/Enzyme:2025/Nov →3. Oligo
- 09:2809:28, 3 November 2025 diff hist −2 XIE/Enzyme:2025/Nov →4.反应
- 09:2809:28, 3 November 2025 diff hist +4 XIE/Enzyme:2025/Nov →4.反应
- 09:2709:27, 3 November 2025 diff hist +206 XIE/Enzyme:2025/Nov →日期 11.3
- 08:4608:46, 3 November 2025 diff hist −2 XIE/Enzyme:2025/Nov →日期 11.3
- 08:4608:46, 3 November 2025 diff hist +114 XIE/Enzyme:2025/Nov No edit summary
- 08:4508:45, 3 November 2025 diff hist +735 N XIE/Enzyme:2025/Nov Created page with "== 日期 11.3 == === 1. GDH === 取 '''GDH 1.4 mg'''，溶解于 '''2 mL PBS (1×)''' 中，形成 '''20 μM''' 溶液。 分装于离心管（每管 '''30 μL'''，共 '''4 管'''），以及 EP 管（'''400 μL、600 μL、800 μL''' 各一管）。 取其中一管，加入 '''90 mL PBS (1×, 5 μM)'''。 取 '''100 μL''' 作为待反应液，剩余 '''20 μL''' 作为凝胶对照液。 === 2. DBCO === 称取 '''DBCO 5 mg'''，加入 '''50 μL DMF (100 mM)''..."
- 08:3808:38, 3 November 2025 diff hist −2 Protocol →5. 向 GDH 样品液中加入 DBCO，并在室温下孵育 4 h
- 08:3808:38, 3 November 2025 diff hist −1 Protocol →4. 稀释 DBCO（样品液）
1 November 2025
- 05:4105:41, 1 November 2025 diff hist +18 Protocol →8. 凝胶电泳
- 05:4005:40, 1 November 2025 diff hist −1 Protocol →7. Oligo
- 05:2405:24, 1 November 2025 diff hist −3 Protocol →7. 加入 Oligo，并在 37 ℃ 下孵育 4 h
- 05:2105:21, 1 November 2025 diff hist 0 Protocol →7. Oligo
- 05:2005:20, 1 November 2025 diff hist −5 Protocol →7. Oligo
- 05:1905:19, 1 November 2025 diff hist +303 Protocol →6. 使用 10 kDa 超滤管超滤去除游离 DBCO
- 05:1105:11, 1 November 2025 diff hist −1 Protocol →7. 加入 Oligo，并在 37 ℃ 下孵育 4 h
- 04:5004:50, 1 November 2025 diff hist +6 Protocol →3. 溶解 DBCO-PEG4-Bis-Sulfone 于 DMF(N,N-二甲基甲酰胺) 中（100 mM）
- 04:5004:50, 1 November 2025 diff hist 0 Protocol →3. 溶解 DBCO-PEG4-Bis-Sulfone 于 DMF(N,N-二甲基甲酰胺) 中（100 mM）
- 04:4904:49, 1 November 2025 diff hist −1 Protocol →3. 溶解 DBCO-PEG4-Bis-Sulfone 于 DMF(N,N-二甲基甲酰胺) 中（100 mM）
- 04:4904:49, 1 November 2025 diff hist +42 Protocol →3. 溶解 DBCO-PEG4-Bis-Sulfone 于 DMF(N,N-二甲基甲酰胺) 中（100 mM）
- 04:4404:44, 1 November 2025 diff hist 0 Protocol →1. GDH 制备
31 October 2025
- 10:2910:29, 31 October 2025 diff hist −4 Protocol →1. GDH 制备