User contributions for Xie guangneng

08:2208:22, 31 October 2025 diff hist −2,866‎ XIE/EnZyme:2025/Oct ‎ Blanked the page current Tag: Blanking
08:2208:22, 31 October 2025 diff hist +2,866‎ N Protocol ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con..."
08:2108:21, 31 October 2025 diff hist +12‎ XIE:Project ‎ →‎Project 1: Protein Conjugation to DNA Origami

12:0512:05, 30 October 2025 diff hist 0‎ Background ‎ →‎一、传统的T细胞激活 current
12:0512:05, 30 October 2025 diff hist +1‎ Background ‎ →‎3. 细胞因子 (Signal 3)
12:0512:05, 30 October 2025 diff hist +63‎ Background ‎ →‎一、传统的T细胞激活
12:0412:04, 30 October 2025 diff hist 0‎ N File:T cell.png ‎No edit summary current
11:1811:18, 30 October 2025 diff hist −6‎ XIE:Project ‎ →‎CAR-T
11:1711:17, 30 October 2025 diff hist −995‎ Information ‎ Blanked the page current Tag: Blanking
11:1711:17, 30 October 2025 diff hist −1,671‎ Information ‎ →‎一、传统的T细胞激活
11:1711:17, 30 October 2025 diff hist +2,666‎ N Background ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 一、传统的T细胞激活 == === 1. 抗原识别 (Signal 1) === ==== 1.1 ==== '''APC（抗原呈递细胞）'''：吞噬/摄取抗原，并向 '''T细胞呈递MHC分子'''（signal 1），并提供'''共刺激分子 B7 (signal 2)'''。 '''TCR（T细胞表面）'''：特异性识别 MHC 分子呈递的抗原肽，与 '''CD3复合体'''（CD3γ, δ,..."
11:1611:16, 30 October 2025 diff hist +16‎ XIE:Project ‎ →‎CAR-T
11:1611:16, 30 October 2025 diff hist −6‎ XIE:Project ‎ →‎Background
11:1511:15, 30 October 2025 diff hist +5‎ XIE:Project ‎ →‎CAR-T
11:1211:12, 30 October 2025 diff hist −13‎ 设计工具 ‎ →‎步骤 current
11:1111:11, 30 October 2025 diff hist −43‎ 设计工具 ‎ →‎代码
11:1111:11, 30 October 2025 diff hist +822‎ N 设计工具 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Wire frame == [https://dnaforge.org DNAforge 官方网站] === 步骤 === '''3D 图形 → 路径渲染 → 圆柱体渲染 → 核酸渲染''' === OxDNA 模拟 === * 打开 '''Docker''' * 在控制面板中输入以下命令： # ls # cd dnaforge-backend # docker compose up -d == 紧密型 == '''cadnano2''' == 步骤 == '''蜂窝/方型绘制..."
11:0811:08, 30 October 2025 diff hist −13‎ XIE:Project ‎ →‎DNA Origami
11:0711:07, 30 October 2025 diff hist −398‎ Wireframe ‎ Blanked the page current Tag: Blanking
10:4310:43, 30 October 2025 diff hist 0‎ Wireframe ‎ →‎步骤
10:4310:43, 30 October 2025 diff hist +5‎ Wireframe ‎ →‎步骤
10:4310:43, 30 October 2025 diff hist +2‎ Wireframe ‎ →‎步骤
10:4210:42, 30 October 2025 diff hist +2‎ Wireframe ‎ →‎步骤
10:4210:42, 30 October 2025 diff hist +4‎ Wireframe ‎ →‎OxDNA 模拟
10:4110:41, 30 October 2025 diff hist −46‎ Wireframe ‎ →‎OxDNA 模拟
10:4110:41, 30 October 2025 diff hist +98‎ Wireframe ‎No edit summary
10:4110:41, 30 October 2025 diff hist +120‎ Wireframe ‎No edit summary
10:2710:27, 30 October 2025 diff hist −10‎ XIE:Project ‎No edit summary
10:2610:26, 30 October 2025 diff hist +5‎ XIE:Project ‎ →‎实验学习
10:2510:25, 30 October 2025 diff hist −1‎ XIE:Project ‎No edit summary
10:2410:24, 30 October 2025 diff hist −184‎ XIE:Project ‎No edit summary
10:2310:23, 30 October 2025 diff hist +114‎ XIE:Project ‎No edit summary
10:2310:23, 30 October 2025 diff hist +2,866‎ N XIE/EnZyme:2025/Oct ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con..."
10:2210:22, 30 October 2025 diff hist +84‎ XIE:Project ‎ →‎Project 1: Protein Conjugation to DNA Origami

09:0109:01, 22 October 2025 diff hist −1‎ XIE:2025/Oct ‎ →‎5. 向 GDH 样品液中加入 DBCO，并在室温下孵育 4 h current
08:5808:58, 22 October 2025 diff hist −1‎ XIE:2025/Oct ‎ →‎Protocol (版本 1.0)
08:5008:50, 22 October 2025 diff hist +2‎ XIE:2025/Oct ‎ →‎5. 向 GDH 样品液中加入 DBCO，并在室温下孵育 4 h
08:5008:50, 22 October 2025 diff hist +3‎ XIE:2025/Oct ‎ →‎7. 加入 Oligo，并在 37 ℃ 下孵育 4 h
08:4908:49, 22 October 2025 diff hist +2‎ XIE:2025/Oct ‎ →‎5. 向 GDH 样品液中加入 DBCO，并在室温下孵育 4 h
08:4908:49, 22 October 2025 diff hist 0‎ XIE:2025/Oct ‎ →‎2. 稀释 GDH（样品液）
08:4908:49, 22 October 2025 diff hist −1‎ XIE:2025/Oct ‎ →‎1. GDH 制备
07:5107:51, 22 October 2025 diff hist +23‎ XIE:2025/Oct ‎ →‎3. 溶解 DBCO-PEG4-Bis-Sulfone 于 DMF 中（100 mM）
07:4507:45, 22 October 2025 diff hist +17‎ XIE:2025/Oct ‎ →‎8. 凝胶电泳
07:4207:42, 22 October 2025 diff hist −24‎ XIE:2025/Oct ‎ →‎Protocol (版本 1.0)
07:4107:41, 22 October 2025 diff hist −3‎ XIE:2025/Oct ‎ →‎Protocol (版本 1.0)

14:1914:19, 21 October 2025 diff hist −13‎ Information ‎ →‎2. 共刺激 (Signal 2)
14:1714:17, 21 October 2025 diff hist −83‎ Information ‎ →‎2. 共刺激 (Signal 2)
11:5411:54, 21 October 2025 diff hist +23‎ Information ‎ →‎1. 抗原识别 (Signal 1)
11:3111:31, 21 October 2025 diff hist −4‎ Information ‎ →‎二、典型的 CAR-T
11:3111:31, 21 October 2025 diff hist +11‎ Information ‎ →‎2. 激活

31 October 2025

30 October 2025

22 October 2025

21 October 2025