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• 07:21, 11 December 2025 Xie guangneng talk contribs created page Protocol 2 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protocol == === pH ==== *'''Stock Solution''': 1 mM *'''Buffer''': 0.5X TBE with 11 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5) *'''Incubation''': 37℃;C, 3 h {| class="wikitable" ! T-S1 ! T-S2 ! TFO ! Buffer ! pH |- | 4 μL | 4 μL | 4 μL | 388 μL | 5.5, 6.5, 7.0, 7.5, 8.5 |} *Fluorometer")