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• 02:48, 4 December 2025 Hu yang talk contribs created page HU:Protocols/细胞培养 (Created page with "===Jurkat cell culture=== #Origin： Chen Dong #Cell resuscitation: two preservation tubes in one T25 bottle（the growth of Jurkat cells is dependent on the density） #condition：suspension culture with 1640 +++ or 1640 ++-（for administering），5 ml system in T25 Cell culture flask， #medium alteration: 24h, remove 1~2mL medium and add 1~2ml fresh 1640(half alteration) #Cell passage：1/2 flask, about 2~3days once(half alteration). About 5 days,whole alteration:...")