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- 13:46, 2 December 2025 Chen dong talk contribs created page CHEN:PROJECT1/2025/Nov (Created page with "===verification of DNA-ligand conjugation via click chemistry=== #15% urea page #sample loading volume: DNA-100μM-2μL+3μL deionized water + 1 μL DNA loading buffer DNA-ligand-50μM-4μL+6μL deionized water + 2 μL DNA loading buffer #key points of protocol ##Dissolve materis in water at 37℃ except 10% APS and TEMED ##samples mixed with DNA loading buffer were heated at 95 ℃ for 5 min and immediately cooled on ice for 2 min")