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• 14:35, 13 January 2026 Xie guangneng talk contribs created page XIE/2:2026/Jan (Created page with "==1.13== ===抗体还原=== *取2 ul(1mM) TCEP,用PBS(7.4)稀释至 20 ul(100uM),取5 ul稀释后的TCEP，加入85 ul PBS(7.40)，涡旋混匀---TCEP溶液pH过低提前中和 *取2 ul(50uM)抗体，用PBS（pH 7.4）稀释至10 ul（10uM），将TECP的PBS缓冲液加入其中，37℃孵育2h. *取1 ul(50uM)抗体，用PBS（pH7.4）稀释至8 ul，作为 C 组 ==偶联=== *将孵育好的溶液用1XPBS(6.5)洗涤4次（10000g 3.5min），均分样品（A.B）----buffe...")