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• 11:04, 4 September 2025 Hu yang talk contribs created page PCR扩增 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ===原理=== <ol> <li><strong>PCR</strong>:通常指的是普通PCR，以双链DNA为模板，以dNTP为底物，定性扩增双链DNA。大致可分为高温变性、低温退火、适温延伸三个基本反应步骤。 <li><strong>qPCR或Real-time PCR</strong>:指的是实时荧光定量PCR，以cDNA为模板，以dNTP 为底物，对扩增出的DNA进行<strong>相对定...")