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• 13:05, 8 July 2025 Hu yang talk contribs created page 细胞提取及分选 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==提取T细胞== #断颈处死小鼠，整体浸入含酒精的烧杯中灭菌3-5min，沿着腹部中线剪开取出脾脏，放入含PBS的6孔板中，转移至细胞房。 #换用新的6孔板或者50mlep管子(容纳筛网0.45），先加3 mL的2%FBS-PBS，放入筛网和脾脏，用针筒芯研磨至无明显固体 #转入灭菌EP管。离心2k rpm 3-5min，弃去上...")