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• 02:53, 5 July 2025 Hu yang talk contribs created page 细胞分选 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> == 操作 == #取组织： ##断颈处死小鼠，整体浸入含酒精的烧杯中灭菌3-5min； ##沿着腹部中线剪开取出脾脏，放入含PBS的6孔板中，转移至细胞房。 #研磨： ## 换用新的6孔板或50ml EP管（用于容纳筛网0.45μm） ## 加3mL的2%FBS-PBS，放入筛网和脾脏，用针筒芯研磨至无明显固体，转入灭菌EP管，离...")