DNA偶合物构建思路: Difference between revisions
(Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == protein == === gene engineering and click chemistry === *'''''Click Chemistry：通过叠氮基团和炔基(环炔)环加成生成三唑环结构''''' *'''''构建生物正交系统：<br>1.在目标蛋白末端基因(N端)插入琥珀密码子(TAG-终止密码子)<br>2.在培养系统中引入特定的tRNA(特异性识别TAG位点)与其对应的氨酰-tR...") |
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Revision as of 15:10, 8 October 2025
protein
gene engineering and click chemistry
- Click Chemistry：通过叠氮基团和炔基(环炔)环加成生成三唑环结构
- 构建生物正交系统：
1.在目标蛋白末端基因(N端)插入琥珀密码子(TAG-终止密码子)
2.在培养系统中引入特定的tRNA(特异性识别TAG位点)与其对应的氨酰-tRNA合成酶(能将p-Azidophenylalanine(含有一个叠氮基团)这个非天然氨基酸连接到tRNA上)
3.BCN-DNA（BNN含环炔)