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== 阶段三 转膜 == *'''剪下合适大小PVDF膜,甲醇浸泡3min,膜的右上角剪一下,用于判断方向,放入缓冲液(凝胶在转膜液中浸泡5min,用于平衡离子)''' *'''''转膜(黑下白上):海绵垫+3层滤纸+PVDF膜+凝胶+3层滤纸+海绵垫+黑色板(放置时黑对黑)''''' *'''4℃,3000 mA转膜60 min(需要大量冰袋,碎冰)等待时配制TBST,5%脱脂奶'''
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