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  • 02:12, 8 December 2025 ‎TEM (hist | edit) ‎[1,129 bytes] ‎Xie guangneng (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Sample Preparation == *Thaw 400 uL of 2% Uranyl-formate (aliqouted in brown tube), then add 8.0 ul of 1M NaOH to the wall of tube and immediately vortex. Centrifuge for 5 min at 16000 rcf, when use, take supernatant to avoid any precipitant. ===Prepare grids=== *Put grids on parafilm wrapped glass-slides Glow-discharge them for 30sec with Plasma Cleaner (t...")
  • 04:52, 6 December 2025 ‎XU1:2025/Dec (hist | edit) ‎[3,359 bytes] ‎Xu anran (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <html> <head> <meta charset="UTF-8"> <title>组会汇报</title> </head> <body> <div class="article"> <div class="date">2025年12月3日</div> <h1 class="title">制备TNF-SA-DNA</h1> <div class="content"> <p>1.样品稀释：生物素化TNF-α储存液 (63.4KDa 1µM)：20 µg粉末溶于317 µLdd水，分装出5管30ul冻存</p...")
  • 07:48, 1 December 2025 ‎Hexagonal Prism DNA Origami (hist | edit) ‎[535 bytes] ‎Xie guangneng (talk | contribs) (Created page with "== Material == === Scaffold === #'''P8064''' '''Powder and Stock Solution at 150 nM''' === Staples === *'''Stock solutions 1 (Hexagonal-prism DNA origami) in three 96‑well plates at 100 uM''' #'''Schematic Diagram''' thumb|center|400px|Schematic Diagram For detailed information, please refer to the shared "Origami" file on Feishu *'''Stock solutions 2 (Unmodified hexagonal‑prism DNA origami) in EP tube at 500 nM'''")
  • 13:19, 25 November 2025 ‎Construction of DNA-Origami (hist | edit) ‎[1,477 bytes] ‎Xie guangneng (talk | contribs) (Created page with "== Construction Material == === Scaffold === #'''P8064''' '''Powder and Stock Solution at 200 nM''' === Staples === #'''Stock solutions in three 96‑well plates at 100 nM''' *'''Schematic Diagram''' File:Staples|thuemb|")
  • 08:45, 3 November 2025 ‎XIE/Enzyme:2025/Nov (hist | edit) ‎[7,699 bytes] ‎Xie guangneng (talk | contribs) (Created page with "== 日期 11.3 == === 1. GDH === 取 '''GDH 1.4 mg'''，溶解于 '''2 mL PBS (1×)''' 中，形成 '''20 μM''' 溶液。 分装于离心管（每管 '''30 μL'''，共 '''4 管'''），以及 EP 管（'''400 μL、600 μL、800 μL''' 各一管）。 取其中一管，加入 '''90 mL PBS (1×, 5 μM)'''。 取 '''100 μL''' 作为待反应液，剩余 '''20 μL''' 作为凝胶对照液。 === 2. DBCO === 称取 '''DBCO 5 mg'''，加入 '''50 μL DMF (100 mM)''...")
  • 08:22, 31 October 2025 ‎Protocol (hist | edit) ‎[5,307 bytes] ‎Xie guangneng (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con...")
  • 11:17, 30 October 2025 ‎Background (hist | edit) ‎[2,730 bytes] ‎Xie guangneng (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 一、传统的T细胞激活 == === 1. 抗原识别 (Signal 1) === ==== 1.1 ==== '''APC（抗原呈递细胞）'''：吞噬/摄取抗原，并向 '''T细胞呈递MHC分子'''（signal 1），并提供'''共刺激分子 B7 (signal 2)'''。 '''TCR（T细胞表面）'''：特异性识别 MHC 分子呈递的抗原肽，与 '''CD3复合体'''（CD3γ, δ,...")
  • 11:11, 30 October 2025 ‎设计工具 (hist | edit) ‎[766 bytes] ‎Xie guangneng (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Wire frame == [https://dnaforge.org DNAforge 官方网站] === 步骤 === '''3D 图形 → 路径渲染 → 圆柱体渲染 → 核酸渲染''' === OxDNA 模拟 === * 打开 '''Docker''' * 在控制面板中输入以下命令： # ls # cd dnaforge-backend # docker compose up -d == 紧密型 == '''cadnano2''' == 步骤 == '''蜂窝/方型绘制...")
  • 10:23, 30 October 2025 ‎XIE/EnZyme:2025/Oct (hist | edit) ‎[0 bytes] ‎Xie guangneng (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con...")
  • 10:46, 21 October 2025 ‎Information (hist | edit) ‎[0 bytes] ‎Xie guangneng (talk | contribs) (Created page with "== 一、传统的T细胞激活 == === 1. 抗原识别 (Signal 1) === ==== 1.1 ==== '''APC（抗原呈递细胞）'''：吞噬/摄取抗原，并向 '''T细胞呈递MHC分子'''（signal 1），并提供'''共刺激分子 B7 (signal 2)'''。 '''TCR（T细胞表面）'''：特异性识别 MHC 分子呈递的抗原肽，与 '''CD3复合体'''（CD3γ, δ, ε, ζ）组成 '''TCR复合体'''。 CD3ζ上的 '''ITAM (immunoreceptor tyrosine-based activation motifs)''' 被...")
  • 15:10, 8 October 2025 ‎DNA偶合物构建思路 (hist | edit) ‎[1,405 bytes] ‎Xie guangneng (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == protein == === gene engineering and click chemistry === *'''''Click Chemistry：通过叠氮基团和炔基(环炔)环加成生成三唑环结构''''' *'''''构建生物正交系统：<br>1.在目标蛋白末端基因(N端)插入琥珀密码子(TAG-终止密码子)<br>2.在培养系统中引入特定的tRNA(特异性识别TAG位点)与其对应的氨酰-tR...")
  • 02:57, 7 October 2025 ‎Protein extraction (hist | edit) ‎[1,029 bytes] ‎Xie guangneng (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 细胞总蛋白提取 == *'''配液：细胞裂解液=PIRP:PMSF=100:1, Loading buffer 蛋白上样缓冲液(5×), β-巯基乙醇(保护巯基)''' *'''提取：")
  • 08:22, 6 October 2025 ‎West Blot (hist | edit) ‎[4,320 bytes] ‎Xie guangneng (talk | contribs) (Created page with "蛋白提取及WB")
  • 08:10, 6 October 2025 ‎Origami (hist | edit) ‎[797 bytes] ‎Xie guangneng (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> === DNA Origami === center|thumb|300px|DNA Origami综述 ''' 一篇关于DNA Origami的综述，列出了三代Origami的设计软件，主要针对折叠、原子模拟，其中可以下载Adential插件的SAMSON，可以模拟含蛋白质、药物分子 的DNA折纸''' === DNA forge === center|thumb|300px|DNA Origami综述 '...")
  • 08:02, 6 October 2025 ‎Engineered Microbes (hist | edit) ‎[415 bytes] ‎Xie guangneng (talk | contribs) (Created page with "=== Engineerd Microbes and optoelectronic capsules === center|thumb|300px|光电微生物 ''' 讲述了构架光电微生物 EcN-NO-Lux 和 EcN-Opto-NbTNF，用于探究肠道炎症标志物硝酸盐，并光激活促进NbTNF产生释放'''")
  • 07:17, 16 September 2025 ‎详情 (hist | edit) ‎[255 bytes] ‎Xu anran (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> center|thumb|300px|")
  • 06:57, 16 September 2025 ‎Wireframe (hist | edit) ‎[0 bytes] ‎Xie guangneng (talk | contribs) (Created page with "== wireframe == <html> <details> <summary style="font-size: 20px; font-weight: bold;">📁 </summary> </html> <div style="border: 1px solid #ccc; padding: 10px; background-color: #f8f8f8;"> '''DNAforge''' </div>")
  • 12:17, 15 September 2025 ‎DNAforge (hist | edit) ‎[538 bytes] ‎Xu anran (talk | contribs) (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==流式测定== #在进行细胞分选前后，均移取部分细胞悬液，加入CD8+抗体，用于后续流式测定T cell的占比，考察试剂盒分选效率 #在加入CFSE溶液前后，均移取部分T cell悬液，流式测定cell CFSE信号，用作cell传代的阴性和阳性对照。 #72h后流式测定细胞传代 File:CFSE荧光强度随细胞分裂递减...")
  • 12:06, 15 September 2025 ‎HU:B/AAAA (hist | edit) ‎[5 bytes] ‎Hu yang (talk | contribs) (Created page with "SDSDF") originally created as "AA"
  • 12:01, 15 September 2025 ‎AADD (hist | edit) ‎[8 bytes] ‎Hu yang (talk | contribs) (Created page with "SHBDJHKS")
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