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Combined display of all available logs of yangwa. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 08:43, 13 December 2025 Xie guangneng talk contribs created page XIE/2:2025/Dec (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 12.12 == *<big>'''<span style="color:red;">Triplex DNA pH Test</span>'''<big> === Sample preparation === *ssDNA:s1(3'Cy5)、S2、TFO(3'Cy3)、S-S1(3'Cy5)、S-S2、S-TFO（3'Cy3）各用纯水配成 1mM. *Buffer:PBS:5.5、6.5、7.0、7.5、8.5(PH) 各 50 ml. === Incubation === *分组：每个 pH 下设立两组（1、2），每组先各加入194 ul 对应...")
- 11:53, 12 December 2025 Xie guangneng talk contribs created page File:TEM Origami(NO).jpg
- 11:53, 12 December 2025 Xie guangneng talk contribs uploaded File:TEM Origami(NO).jpg
- 07:21, 11 December 2025 Xie guangneng talk contribs created page Protocol 2 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protocol == === pH ==== *'''Stock Solution''': 1 mM *'''Buffer''': 0.5X TBE with 11 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5) *'''Incubation''': 37℃;C, 3 h {| class="wikitable" ! T-S1 ! T-S2 ! TFO ! Buffer ! pH |- | 4 μL | 4 μL | 4 μL | 388 μL | 5.5, 6.5, 7.0, 7.5, 8.5 |} *Fluorometer")
- 10:01, 10 December 2025 Xie guangneng talk contribs created page File:Origami(NO) 25 12 10.png
- 10:01, 10 December 2025 Xie guangneng talk contribs uploaded File:Origami(NO) 25 12 10.png
- 08:56, 10 December 2025 Xie guangneng talk contribs created page XIE/Enzyme:2025/Dec (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 12")
- 02:12, 8 December 2025 Xie guangneng talk contribs created page TEM (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Sample Preparation == *Thaw 400 uL of 2% Uranyl-formate (aliqouted in brown tube), then add 8.0 ul of 1M NaOH to the wall of tube and immediately vortex. Centrifuge for 5 min at 16000 rcf, when use, take supernatant to avoid any precipitant. ===Prepare grids=== *Put grids on parafilm wrapped glass-slides Glow-discharge them for 30sec with Plasma Cleaner (t...")
- 07:51, 1 December 2025 Xie guangneng talk contribs created page File:Oligo.xlsx
- 07:51, 1 December 2025 Xie guangneng talk contribs uploaded File:Oligo.xlsx
- 07:49, 1 December 2025 Xie guangneng talk contribs created page File:YangLab HR staples.xlsx
- 07:49, 1 December 2025 Xie guangneng talk contribs uploaded File:YangLab HR staples.xlsx
- 07:48, 1 December 2025 Xie guangneng talk contribs created page Hexagonal Prism DNA Origami (Created page with "== Material == === Scaffold === #'''P8064''' '''Powder and Stock Solution at 150 nM''' === Staples === *'''Stock solutions 1 (Hexagonal-prism DNA origami) in three 96‑well plates at 100 uM''' #'''Schematic Diagram''' thumb|center|400px|Schematic Diagram For detailed information, please refer to the shared "Origami" file on Feishu *'''Stock solutions 2 (Unmodified hexagonal‑prism DNA origami) in EP tube at 500 nM'''")
- 07:44, 1 December 2025 Xie guangneng talk contribs created page Yangwa:SharedResources (Created page with "aaaaa")
- 12:33, 30 November 2025 Xie guangneng talk contribs created page File:25 11 27.png
- 12:33, 30 November 2025 Xie guangneng talk contribs uploaded File:25 11 27.png
- 08:19, 27 November 2025 Xie guangneng talk contribs created page File:25 11 26 2.png
- 08:19, 27 November 2025 Xie guangneng talk contribs uploaded File:25 11 26 2.png
- 08:15, 27 November 2025 Xie guangneng talk contribs created page File:25 11 26 1.png
- 08:15, 27 November 2025 Xie guangneng talk contribs uploaded File:25 11 26 1.png
- 13:21, 25 November 2025 Xie guangneng talk contribs created page File:Staples.png
- 13:21, 25 November 2025 Xie guangneng talk contribs uploaded File:Staples.png
- 13:19, 25 November 2025 Xie guangneng talk contribs created page Construction of DNA-Origami (Created page with "== Construction Material == === Scaffold === #'''P8064''' '''Powder and Stock Solution at 200 nM''' === Staples === #'''Stock solutions in three 96‑well plates at 100 nM''' *'''Schematic Diagram''' File:Staples|thuemb|")
- 10:09, 17 November 2025 Xie guangneng talk contribs created page XIE:2025/Dec (Created page with "XIE:Project 1: Protein Conjugation to DNA Origami/protocol")
- 10:32, 14 November 2025 Xie guangneng talk contribs created page File:RuCHMO S2 11 13.jpg
- 10:32, 14 November 2025 Xie guangneng talk contribs uploaded File:RuCHMO S2 11 13.jpg
- 10:29, 14 November 2025 Xie guangneng talk contribs created page File:RuCHMO S2 11 11.jpg
- 10:29, 14 November 2025 Xie guangneng talk contribs uploaded File:RuCHMO S2 11 11.jpg
- 12:04, 6 November 2025 Xie guangneng talk contribs uploaded a new version of File:25 11 6 GDH S1.jpg
- 12:02, 6 November 2025 Xie guangneng talk contribs created page File:25 11 6 GDH S1.jpg
- 12:02, 6 November 2025 Xie guangneng talk contribs uploaded File:25 11 6 GDH S1.jpg
- 02:34, 5 November 2025 Xie guangneng talk contribs created page File:Page1.jpg
- 02:34, 5 November 2025 Xie guangneng talk contribs uploaded File:Page1.jpg
- 12:24, 4 November 2025 Xie guangneng talk contribs created page File:Reference of gel concentration.jpg
- 12:24, 4 November 2025 Xie guangneng talk contribs uploaded File:Reference of gel concentration.jpg
- 08:45, 3 November 2025 Xie guangneng talk contribs created page XIE/Enzyme:2025/Nov (Created page with "== 日期 11.3 == === 1. GDH === 取 '''GDH 1.4 mg'''，溶解于 '''2 mL PBS (1×)''' 中，形成 '''20 μM''' 溶液。 分装于离心管（每管 '''30 μL'''，共 '''4 管'''），以及 EP 管（'''400 μL、600 μL、800 μL''' 各一管）。 取其中一管，加入 '''90 mL PBS (1×, 5 μM)'''。 取 '''100 μL''' 作为待反应液，剩余 '''20 μL''' 作为凝胶对照液。 === 2. DBCO === 称取 '''DBCO 5 mg'''，加入 '''50 μL DMF (100 mM)''...")
- 08:22, 31 October 2025 Xie guangneng talk contribs created page Protocol (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con...")
- 12:04, 30 October 2025 Xie guangneng talk contribs created page File:T cell.png
- 12:04, 30 October 2025 Xie guangneng talk contribs uploaded File:T cell.png
- 11:17, 30 October 2025 Xie guangneng talk contribs created page Background (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == 一、传统的T细胞激活 == === 1. 抗原识别 (Signal 1) === ==== 1.1 ==== '''APC（抗原呈递细胞）'''：吞噬/摄取抗原，并向 '''T细胞呈递MHC分子'''（signal 1），并提供'''共刺激分子 B7 (signal 2)'''。 '''TCR（T细胞表面）'''：特异性识别 MHC 分子呈递的抗原肽，与 '''CD3复合体'''（CD3γ, δ,...")
- 11:11, 30 October 2025 Xie guangneng talk contribs created page 设计工具 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Wire frame == [https://dnaforge.org DNAforge 官方网站] === 步骤 === '''3D 图形 → 路径渲染 → 圆柱体渲染 → 核酸渲染''' === OxDNA 模拟 === * 打开 '''Docker''' * 在控制面板中输入以下命令： # ls # cd dnaforge-backend # docker compose up -d == 紧密型 == '''cadnano2''' == 步骤 == '''蜂窝/方型绘制...")
- 10:23, 30 October 2025 Xie guangneng talk contribs created page XIE/EnZyme:2025/Oct (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con...")
- 10:46, 21 October 2025 Xie guangneng talk contribs created page Information (Created page with "== 一、传统的T细胞激活 == === 1. 抗原识别 (Signal 1) === ==== 1.1 ==== '''APC（抗原呈递细胞）'''：吞噬/摄取抗原，并向 '''T细胞呈递MHC分子'''（signal 1），并提供'''共刺激分子 B7 (signal 2)'''。 '''TCR（T细胞表面）'''：特异性识别 MHC 分子呈递的抗原肽，与 '''CD3复合体'''（CD3γ, δ, ε, ζ）组成 '''TCR复合体'''。 CD3ζ上的 '''ITAM (immunoreceptor tyrosine-based activation motifs)''' 被...")
- 09:10, 16 October 2025 Xie guangneng talk contribs created page XIE:2025/Oct (Created page with "== Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === *'''''Conjugation: Proteins containing 6x Histidine at the C terminus reacted, with the chemical Bis-sulfone-DBCO for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is conjugated to the protein by click chemistry between the azide- and the DBCO-group.'''' *'''sequence(RuCHMO):CTCTCCTTCTTCCCTTTCTTT''''...")
- 15:35, 8 October 2025 Xie guangneng talk contribs created page File:锌指蛋白 DNA.png
- 15:35, 8 October 2025 Xie guangneng talk contribs uploaded File:锌指蛋白 DNA.png
- 15:29, 8 October 2025 Xie guangneng talk contribs created page File:生物正交.png
- 15:29, 8 October 2025 Xie guangneng talk contribs uploaded File:生物正交.png
- 15:26, 8 October 2025 Xie guangneng talk contribs created page File:Click Chemistry.jpg
- 15:26, 8 October 2025 Xie guangneng talk contribs uploaded File:Click Chemistry.jpg
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