Protocol 2

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Protocol[edit]

pH[edit]

  • Stock Solution: 1 mM
  • Buffer: 0.5X TBE with 10 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5)
  • Incubation: 37℃;C, 3 h
T-S1 T-S2 TFO Buffer pH
4 μL 4 μL 4 μL 388 μL 5.5 , 6.5 , 7.0 , 7.5 , 8.5
  • Fluorometer
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