All public logs
Combined display of all available logs of yangwa. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 03:07, 9 January 2026 Hu yang talk contribs uploaded File:HU PROJECT1 20260108 lentivirus infection on HEK 293T.png
- 01:46, 5 January 2026 Hu yang talk contribs created page File:Hu-PROJECT-1 20260106 psPAX2 plasmid transfering.png
- 01:46, 5 January 2026 Hu yang talk contribs uploaded File:Hu-PROJECT-1 20260106 psPAX2 plasmid transfering.png
- 13:07, 4 January 2026 Hu yang talk contribs created page File:HUproject2-1214质粒转化pFUGW，pFUGW mkate.png
- 13:07, 4 January 2026 Hu yang talk contribs uploaded File:HUproject2-1214质粒转化pFUGW，pFUGW mkate.png
- 03:20, 3 January 2026 Hu yang talk contribs created page HU:PROJECT1/2026/Jan (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==20250102 induce FAPα on NIH 3T3（Select the Amount of TGF-β ）== *dmem++- was applied for Exp. group as it is difficult to control the quality of FBS from different batches in DMEM+++ #Group：n=3 ##DMEM +++ ## 0、5、10、15、20 ng/ml in DMEM ++- from TGF-beta(10ug/ml in PBS): <table border="1" cellpadding="8" cellspacing="0" style="width: 100%; text-align: center...")
- 03:11, 3 January 2026 Hu yang talk contribs created page HU:PROJECT2/2026/Jan (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==20250102 resuscite HEK 293 T== #cell line: 293T from 何志迪，293T from 胡杨（20251230保种液保种） #result：293T from 胡杨复苏后贴壁细胞较少，293T from 何志迪复苏后基本全部贴壁 #discussion:保种液？(20251230同时保种液保种了NIH 3T3,能正常复苏)，复苏操作？（水浴时间较长），保种前细胞状态（生长缓慢）")
- 12:53, 30 December 2025 Hu yang talk contribs created page File:Hu-PROJECT-1 20251229 plasmid transfection to HEK293T.png
- 12:53, 30 December 2025 Hu yang talk contribs uploaded File:Hu-PROJECT-1 20251229 plasmid transfection to HEK293T.png
- 12:50, 30 December 2025 Hu yang talk contribs created page File:HU PROJECT1 20251230 FAPα induced by TGFβ for 24h.png
- 12:50, 30 December 2025 Hu yang talk contribs uploaded File:HU PROJECT1 20251230 FAPα induced by TGFβ for 24h.png
- 07:45, 28 December 2025 Hu yang talk contribs created page HU:常用信息/网址 (Created page with " <div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> #蛋白数据库： https://www.fpbase.org/")
- 02:26, 26 December 2025 Hu yang talk contribs created page File:HU PROJECT1-20251225 Ntive Page & FC of 2-CD3-L4.png
- 02:26, 26 December 2025 Hu yang talk contribs uploaded File:HU PROJECT1-20251225 Ntive Page & FC of 2-CD3-L4.png
- 14:20, 24 December 2025 Hu yang talk contribs created page HU:Protocols/常用核酸相关信息 (Created page with "<table border="1" cellpadding="6" cellspacing="0" style="width: 40%; text-align: left;"> <thead> <tr> <<th>IUPAC nucleotide code</</th> <<th>Base</</th> </tr> </thead> <tbody> <tr> <td>A</td> <td>Adenine</td> </tr> <tr> <td>C</td> <td>Cytosine</td> </tr> <tr> <td>G</td> <td>Guanine</td> </tr> <tr> <td>T (or U)</td> <td>Thymine (or Uracil)</td> </tr> <tr> <t...")
- 10:18, 23 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-20251224 FC of 2-CD3-L4.png
- 10:18, 23 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-20251224 FC of 2-CD3-L4.png
- 03:26, 23 December 2025 Hu yang talk contribs created page File:Hu project1120251223 Ntive Page of 2-CD3-L4.png
- 03:26, 23 December 2025 Hu yang talk contribs uploaded File:Hu project1120251223 Ntive Page of 2-CD3-L4.png
- 09:37, 21 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-CD3-ATP aptamer缩写-2nd design.png
- 09:37, 21 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-CD3-ATP aptamer缩写-2nd design.png
- 12:47, 19 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-20251218WB结果图.png
- 12:47, 19 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-20251218WB结果图.png
- 01:19, 15 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-20251214TGF-β给药时间筛选.png
- 01:19, 15 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-20251214TGF-β给药时间筛选.png
- 11:53, 12 December 2025 Hu yang talk contribs created page File:HU-PROJECT2-20251212 pVSV G质粒转化.png
- 11:53, 12 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT2-20251212 pVSV G质粒转化.png
- 07:00, 11 December 2025 Hu yang talk contribs created page HU:PROJECT2/2025/Dec (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ===滤纸质粒提取=== #about 2 µg of plasmid DNA per circle #To recover the DNA, use clean gloves and cut the marked circle area that contains the dried plasmid DNA. #Using clean forceps, insert the filter paper into a 1.5 ml micro centrifuge tube. Add 100 µl of TE buffer, vortex briefly and incubate at room temperature for 5 minutes. Vortex again and centrifuge the tub...")
- 12:31, 4 December 2025 Hu yang talk contribs created page HU:PROJECT1/2025/Dec (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==2nd Selection and Optimization of CD3-ATP Dual-aptamer system== 410px|thumb|center|{{#tag:div|ATP-CD3_Aptamer结合长度筛选|style="color: black;"}} 410px|thumb|center|{{#tag:div|ATP-CD3_Aptamer各序列结构比对|style="color: black;"}} *新增的ATP-L-611开始 结...")
- 11:55, 4 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-Aptamer结构比对-2.png
- 11:55, 4 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-Aptamer结构比对-2.png
- 11:52, 4 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-Aptamer结合长度筛选-2.png
- 11:52, 4 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-Aptamer结合长度筛选-2.png
- 07:31, 4 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-20251202 FC.PNG
- 07:31, 4 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-20251202 FC.PNG
- 07:30, 4 December 2025 Hu yang talk contribs created page File:HU-PROJECT1-20251201 Native Page.PNG
- 07:30, 4 December 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-20251201 Native Page.PNG
- 02:48, 4 December 2025 Hu yang talk contribs created page HU:Protocols/细胞培养 (Created page with "===Jurkat cell culture=== #Origin： Chen Dong #Cell resuscitation: two preservation tubes in one T25 bottle（the growth of Jurkat cells is dependent on the density） #condition：suspension culture with 1640 +++ or 1640 ++-（for administering），5 ml system in T25 Cell culture flask， #medium alteration: 24h, remove 1~2mL medium and add 1~2ml fresh 1640(half alteration) #Cell passage：1/2 flask, about 2~3days once(half alteration). About 5 days,whole alteration:...")
- 12:30, 1 December 2025 Hu yang talk contribs moved page HU:Aabbcc to HU:Aabbccdd
- 12:30, 1 December 2025 Hu yang talk contribs created page HU:Aabbcc (Created page with "sjhdbvkjfvhlkfv")
- 07:54, 1 December 2025 Hu yang talk contribs created page File:Hu-Project1-20251130 Agarose gel.png
- 07:54, 1 December 2025 Hu yang talk contribs uploaded File:Hu-Project1-20251130 Agarose gel.png
- 07:53, 1 December 2025 Hu yang talk contribs created page File:Hu-Project1-20251129 Agarose gel.png
- 07:53, 1 December 2025 Hu yang talk contribs uploaded File:Hu-Project1-20251129 Agarose gel.png
- 03:18, 28 November 2025 Hu yang talk contribs created page File:HU-PROJECT1-CD3-ATP aptamer缩写.png
- 03:18, 28 November 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-CD3-ATP aptamer缩写.png
- 13:23, 25 November 2025 Hu yang talk contribs created page HU:Protocols/细胞周期 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==原理== 碘化丙啶(Propidium，简称PI)是一种双链DNA的荧光染料。碘化丙啶和双链DNA结合后可以产生荧光，并且荧光强度和双链DNA的含量成正比。细胞内的DNA被碘化丙啶染色后，可以用流式细胞仪对细胞进行DNA含量测定，然后根据DNA含量的分布情况，可以进行细胞周期和细胞凋亡分析。 碘...")
- 06:55, 22 November 2025 Hu yang talk contribs created page File:HU-PROJECT1-251121 碧云天-NEB ATP比对.png
- 06:55, 22 November 2025 Hu yang talk contribs uploaded File:HU-PROJECT1-251121 碧云天-NEB ATP比对.png
- 03:36, 20 November 2025 Hu yang talk contribs uploaded a new version of File:HU-Project1-Design of CD3-PDGF Dual-aptamer system.png
