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Combined display of all available logs of yangwa. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 03:38, 11 November 2025 Hu yang talk contribs created page File:HU-Project1-20251108 PDGF aptamer与Jurkat结合结果.png
- 03:38, 11 November 2025 Hu yang talk contribs uploaded File:HU-Project1-20251108 PDGF aptamer与Jurkat结合结果.png
- 03:35, 11 November 2025 Hu yang talk contribs uploaded a new version of File:HU-Project1-20251107dsDNA与Jurkat结合结果.png
- 12:20, 7 November 2025 Hu yang talk contribs uploaded a new version of File:Hu-Protocols-triple helix formation in a DNA frame.png
- 12:14, 7 November 2025 Hu yang talk contribs created page File:Hu-Protocols-triple helix formation in a DNA frame.png
- 12:14, 7 November 2025 Hu yang talk contribs uploaded File:Hu-Protocols-triple helix formation in a DNA frame.png
- 12:05, 7 November 2025 Hu yang talk contribs created page File:Hoogsteen pairing doi 10.1093-nar-gkv241 .png
- 12:05, 7 November 2025 Hu yang talk contribs uploaded File:Hoogsteen pairing doi 10.1093-nar-gkv241 .png
- 11:55, 7 November 2025 Hu yang talk contribs uploaded a new version of File:G-quadruplex-structure.png
- 08:57, 7 November 2025 Hu yang talk contribs created page File:HU-Project1-20251107dsDNA与Jurkat结合结果.png
- 08:57, 7 November 2025 Hu yang talk contribs uploaded File:HU-Project1-20251107dsDNA与Jurkat结合结果.png
- 02:33, 7 November 2025 Hu yang talk contribs created page HU:Protocols/细胞凋亡 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==原理== Annexin V-YSFluor"647/7-AAD细胞调亡检试剂盒是用荧光染料YSFluo 647 (Phycoerythrin)标记的Annexiny作为探针，来检测细胞早期调亡的发生，可用荧光显微镜、流式细胞仅或其他荧光检测设备进行检测。 #凋亡细胞：在正常的活细胞中，磷脂酰丝氨酸(phosphotidylserine,PS)位于细胞膜的内侧，但在...")
- 12:38, 6 November 2025 Hu yang talk contribs created page File:HU-Project1-251106dsDNA与Jurkat结合.png
- 12:38, 6 November 2025 Hu yang talk contribs uploaded File:HU-Project1-251106dsDNA与Jurkat结合.png
- 06:02, 5 November 2025 Hu yang talk contribs created page File:HU-Project1-2510map.xlsx
- 06:02, 5 November 2025 Hu yang talk contribs uploaded File:HU-Project1-2510map.xlsx
- 02:02, 5 November 2025 Hu yang talk contribs uploaded a new version of File:Hu-Project1-CD3-Jurkat cell binding（with random sequence）.jpg
- 01:59, 5 November 2025 Hu yang talk contribs created page File:Hu-Project1-CD3-Jurkat cell binding（with random sequence）.jpg
- 01:59, 5 November 2025 Hu yang talk contribs uploaded File:Hu-Project1-CD3-Jurkat cell binding（with random sequence）.jpg
- 02:02, 31 October 2025 Hu yang talk contribs created page HU:PROJECT1/2025/Nov (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==Reliability of ATP Aptamer== *Formation of signal dsDNA and CD3+ATP dsDNA（after incubation, diluete to 25nM） #37℃ 2h #Groups: ATP Aptamer; Signal DNA;CD3 aptamer;fresh×2; dsDNA×2 *Addition of ATP: ATP dsDNA at 40 nM was incubated in the absence of ATP for 10 min at 37 ℃, followed by the addition of ATP to 1 mM and a further incubation at the same temperature for...")
- 06:33, 29 October 2025 Hu yang talk contribs created page File:HY-20251029-Binding of the cd3 against Jurkat-2.png
- 06:33, 29 October 2025 Hu yang talk contribs uploaded File:HY-20251029-Binding of the cd3 against Jurkat-2.png
- 06:32, 29 October 2025 Hu yang talk contribs created page File:HY-20251029-Binding of the cd3 against Jurkat.png
- 06:32, 29 October 2025 Hu yang talk contribs uploaded File:HY-20251029-Binding of the cd3 against Jurkat.png
- 03:16, 29 October 2025 Hu yang talk contribs uploaded a new version of File:HY Head Photo.jpg
- 10:53, 20 October 2025 Hu yang talk contribs created page File:HY 2510单蛋白孵育及对dsDNA形成的影响.png
- 10:53, 20 October 2025 Hu yang talk contribs uploaded File:HY 2510单蛋白孵育及对dsDNA形成的影响.png
- 06:02, 14 October 2025 Hu yang talk contribs created page HU:常用信息/课程 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==药代动== #三次讨论")
- 09:03, 6 October 2025 Hu yang talk contribs created page HU:PROJECT1/2025/Oct (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> == Method optimization for preparation of dsDNA== <ol> <li>Stock solution：dissolve aptamer to 100 μM by UP water <li>preparation of dsDNA (n=2): add 1 μL CD3e aptamer +1 μL PDGF aptamer +8 μL UP water (10 uM). Method optimization: #preheat and anneal: 95℃ 2min；70℃ 2min；60℃ 10min；50℃ 10min；40℃ 10min；37℃ 10min；RT 10min；4℃ for strorage # 37℃...")
- 08:10, 28 September 2025 Hu yang talk contribs created page HU:Protocols/Delivery of nucleic acid (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==核酸脂质纳米粒LNP== Lipid Nanoparticle #核酸与普通化学药物的一个明显区别是核酸带有数量庞大的磷酸根，因而呈负电。为了使其能够被脂质纳米粒更好地包裹，使用一类特殊的脂质——阳离子脂质。阳离子脂质往往具有一个带铵根的亲水端，在酸性环境下能够与氢离子结合呈正电(NH4+)...")
- 01:33, 27 September 2025 Hu yang talk contribs created page HU:Protocols/He's lab management (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> 〈ol〉 〈/ol〉")
- 08:08, 26 September 2025 Hu yang talk contribs created page File:G-quadruplex-structure.png
- 08:08, 26 September 2025 Hu yang talk contribs uploaded File:G-quadruplex-structure.png
- 02:45, 24 September 2025 Hu yang talk contribs created page HU:Protocols/Design (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *Scaffold lab-made from T4 *Staples better within 60-40nt: available procurement, avoidance of structure crack")
- 02:39, 24 September 2025 Hu yang talk contribs created page HU:Project2/Q&A (Created page with "*the design of promoter from Oxford")
- 08:33, 22 September 2025 Hu yang talk contribs created page HU:常用信息/报账 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==链接== *智慧财务：微信搜索'川大智慧财务'，点击‘服务平台’，常用‘日常报销’（材料费），‘国内差旅’，‘酬金申报’ *智慧财务：四川大学财务处综合信息门户（https://fdinfo.scu.edu.cn/WFManager/login.jsp），点击‘智能报销平台’ *网上预约：四川大学财务处综合信息门户（https://f...")
- 02:36, 20 September 2025 Hu yang talk contribs moved page AAAA to HU:11333
- 02:29, 20 September 2025 Hu yang talk contribs moved page Q&A to HU:Project1/Q&A
- 02:29, 20 September 2025 Hu yang talk contribs moved page Overview to HU:Project1/Overview
- 02:19, 20 September 2025 Hu yang talk contribs moved page 其它 to HU:Protocols/其它
- 02:17, 20 September 2025 Hu yang talk contribs moved page HU:Protocols/PCR to HU:Protocols/质粒
- 02:16, 20 September 2025 Hu yang talk contribs moved page PCR to HU:Protocols/PCR
- 02:16, 20 September 2025 Hu yang talk contribs moved page 纳米粒 to HU:Protocols/纳米粒
- 02:16, 20 September 2025 Hu yang talk contribs moved page Exonuclease I to HU:Protocols/Exonuclease I
- 02:16, 20 September 2025 Hu yang talk contribs moved page PCR扩增 to HU:Protocols/PCR扩增
- 02:15, 20 September 2025 Hu yang talk contribs moved page Native Page to HU:Protocols/Native Page
- 02:15, 20 September 2025 Hu yang talk contribs moved page 琼脂糖凝胶电泳 to HU:Protocols/琼脂糖凝胶电泳
- 02:15, 20 September 2025 Hu yang talk contribs moved page OligoTaC to HU:Protocols/OligoTaC
- 02:15, 20 September 2025 Hu yang talk contribs moved page Ligation to HU:Protocols/Ligation
- 02:13, 20 September 2025 Hu yang talk contribs moved page CCK8 to HU:Protocols/CCK8
