All public logs
Combined display of all available logs of yangwa. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 02:12, 20 September 2025 Hu yang talk contribs moved page 细胞房注意事项 to HU:Protocols/细胞房注意事项
- 02:12, 20 September 2025 Hu yang talk contribs moved page 常用细胞实验操作 to HU:Protocols/常用细胞实验操作
- 02:12, 20 September 2025 Hu yang talk contribs moved page 蛋白提取及WB to HU:Protocols/蛋白提取及WB
- 02:11, 20 September 2025 Hu yang talk contribs moved page 细胞提取及分选 to HU:Protocols/细胞提取及分选
- 02:11, 20 September 2025 Hu yang talk contribs moved page 流式 to HU:Protocols/流式
- 02:10, 20 September 2025 Hu yang talk contribs moved page 长期 to HU:常用信息/长期
- 02:09, 20 September 2025 Hu yang talk contribs moved page 近期 to HU:常用信息/近期
- 02:07, 20 September 2025 Hu yang talk contribs moved page 账号及密码 to HU:常用信息/账号及密码
- 08:21, 19 September 2025 Hu yang talk contribs created page HU:Project1/Q&A-2 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *How do we determine the Aptamers can work？ CD3：After attaching to Jurkat T cell， the Fluorophore From aptamer can be detected by FC<br> PDGF:After attaching to PDGF protein， Agarose gel can distinguish the RNA binding to PDGF or not.(gold view) *afirm Dissociation of dsDNA afer adding PDGF #FRET #Agarose gel:Free CD3 Aptamer increase,PDGF aptamer binding to protein i...")
- 07:38, 18 September 2025 Hu yang talk contribs uploaded a new version of File:HY Head Photo.jpg
- 12:12, 15 September 2025 Hu yang talk contribs moved page AAAA to HU:B/AAAA
- 12:07, 15 September 2025 Hu yang talk contribs moved page AA to AAAA
- 12:06, 15 September 2025 Hu yang talk contribs created page AA (Created page with "SDSDF")
- 12:01, 15 September 2025 Hu yang talk contribs created page AADD (Created page with "SHBDJHKS")
- 02:42, 9 September 2025 Hu yang talk contribs created page Exonuclease I (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *Enzymatic PCR Cleanup Protocol :To remove surplus primers and dNTP for further application <ol> <li>Set-up the reaction as follows: <table class="wikitable" style="margin: auto; border-collapse: collapse; border: 1px solid #a2a9b1;"> <tr> <th>COMPONENTS</th> <th>Reaction Volumes</th> </tr> <tr> <td>PCR Product</td> <td>5 μl</td>...")
- 08:54, 6 September 2025 Hu yang talk contribs created page File:TCR激活（第一信号）.jpg
- 08:54, 6 September 2025 Hu yang talk contribs uploaded File:TCR激活（第一信号）.jpg
- 08:53, 6 September 2025 Hu yang talk contribs created page File:TCR激活（第二信号）.jpg
- 08:53, 6 September 2025 Hu yang talk contribs uploaded File:TCR激活（第二信号）.jpg
- 08:53, 6 September 2025 Hu yang talk contribs created page File:TCR-CD3复合物结构.jpg
- 08:53, 6 September 2025 Hu yang talk contribs uploaded File:TCR-CD3复合物结构.jpg
- 13:31, 5 September 2025 Hu yang talk contribs created page Q&A (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <ol> <li>Q：CD3和CD28的连接比例如何控制 </ol>")
- 13:00, 5 September 2025 Hu yang talk contribs created page File:三特异性抗体原理图2.png
- 13:00, 5 September 2025 Hu yang talk contribs uploaded File:三特异性抗体原理图2.png
- 13:00, 5 September 2025 Hu yang talk contribs created page File:三特异性抗体原理图1.png
- 13:00, 5 September 2025 Hu yang talk contribs uploaded File:三特异性抗体原理图1.png
- 12:49, 5 September 2025 Hu yang talk contribs created page Overview (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;">")
- 13:31, 4 September 2025 Hu yang talk contribs created page 其它 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> #进样小瓶清洗： 泡入乙醇，超声，搽去字迹；自来水清洗；UP水清洗")
- 11:04, 4 September 2025 Hu yang talk contribs created page PCR扩增 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ===原理=== <ol> <li><strong>PCR</strong>:通常指的是普通PCR，以双链DNA为模板，以dNTP为底物，定性扩增双链DNA。大致可分为高温变性、低温退火、适温延伸三个基本反应步骤。 <li><strong>qPCR或Real-time PCR</strong>:指的是实时荧光定量PCR，以cDNA为模板，以dNTP 为底物，对扩增出的DNA进行<strong>相对定...")
- 10:42, 4 September 2025 Hu yang talk contribs created page 长期 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> #DNA Origami综述,DDL:12月 #专利")
- 10:39, 4 September 2025 Hu yang talk contribs created page 近期 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> 20250904 组会 *陈栋师兄课题 #多价Oligotac 21bp循环多价构建（另一侧是否可以保留41nt），价态3 6 #环状126bp，Oligotac单体同时用作splint（10+11） #PCR：购买试剂，包括PCR试剂盒（NEB luna PCR）、DNA外切酶 *三抗 #购买试剂")
- 14:03, 3 September 2025 Hu yang talk contribs created page Native Page (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> 18孔梳，上样量10pmol（1 uM,10uL）")
- 13:45, 3 September 2025 Hu yang talk contribs created page 账号及密码 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==房间密码== *电梯：1234 *PCR，荧光：1348 *WB，酶标板：248965 ==其它== *何组公共实验平台密码：2589")
- 13:08, 3 September 2025 Hu yang talk contribs created page HU:2025/Sep (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *第一周 #细胞铺板 Oligotac制备 脂质体制备 #细胞给药（Oligotac不同价态 脂质体浓度） #WB 流式（脂质体MCHI表达）")
- 09:09, 19 August 2025 Hu yang talk contribs moved page 2222 to 1111
- 09:08, 19 August 2025 Hu yang talk contribs created page 2222 (Created page with "Test")
- 09:36, 18 August 2025 Hu yang talk contribs created page OligoTaC (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==OligoTaC制备== <ol> <li>单链DNA：100 uM in H2O <li>单价Oligotac制备 <ol> <li>制备一价双链DNA：分别取5 ul互补DNA单链，加入10 ul UP水 <li>37度摇床1.5 h <li>RT overnight </ol> <li>多价Oligotac制备 <ol>??? <li>取5ul上诉单价体系，加入多价单链DNA 5 ul <li>37度摇床1.5 h <li>琼脂糖凝胶验证")
- 14:47, 12 August 2025 Hu yang talk contribs created page Ligation (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <ol> <li><strong>Ligation Protocol with T4 DNA Ligase (M0202)</strong></br> <ol> <li>Set up the following reaction in a microcentrifuge tube on ice. ( Note that the table shows a ligation using a <strong>molar ratio</strong> of 1:3 vector to insert for the indicated DNA sizes.) Use NEBioCalculator to calculate molar ratios. <table class="wikitable" style="margin: 0 auto; bo...")
- 13:56, 12 August 2025 Hu yang talk contribs moved page 常用细胞房操作 to HU:1
- 13:53, 12 August 2025 Hu yang talk contribs created page 常用细胞实验操作 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==更换培养基== #更换频率1次/d。 #弃去旧培养基： ##贴壁细胞（如癌细胞）：移液枪直接吸取培养基并弃去； ##悬浮细胞：收集原培养基及cell置于EP管中，离心，弃上清： ##半贴壁细胞：采用半换液，倾斜孔板或培养皿，移液枪吸取上层培养基弃去。 #无需PBS清洗,直接加入新鲜培养基。...")
- 13:40, 12 August 2025 Hu yang talk contribs created page 纳米粒 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <ol> <li> <strong>脂质体</strong> <ol> <li> <strong>胆固醇</strong> <p>胆固醇属于两亲物质，同时具有亲油和亲水两种基团，但是其亲油性较亲水性强，与磷脂相互作用形成脂质体时可嵌入到磷脂分子之间，调节膜结构及性质...")
- 14:21, 11 August 2025 Hu yang talk contribs created page HU:2025/Aug (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *第三周 #cell铺板，脂质体制备，oligo体系合成")
- 06:51, 26 July 2025 Hu yang talk contribs created page 流式 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <big>'''一、操作'''</big> #CD8+ T cell单染PE-H(A) #人源性CD8 T MHCI-I染色：FBS溶液封闭+鼠抗人MHCI+山羊抗鼠荧光抗体 #进样前培养基更换为PBS，避免污染培养基。吸取染色后的cell，PBS稀释，Cell总量约为e6-e5，转移至流式管中500ul样本上样（每次进样仅需约30 ul） #阴性管：只有细胞，没有荧光抗...")
- 03:30, 26 July 2025 Hu yang talk contribs created page CCK8 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *<big>'''一、实验应用'''</big> 1. 细胞增殖测定 2. 细胞毒性测定 3. 细胞活性测定 (不加样本) 4. 药物筛选 5. 肿瘤药敏试验 6. 微生物对细胞毒性或增殖的测定 *<big>'''二、实验原理'''</big> CCK8全称Cell Counting Kit-8试剂，可用于简便而准确的细胞增殖和毒性分析。其基本原理为：该试剂中含有水...")
- 13:44, 22 July 2025 Hu yang talk contribs created page PCR (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> #质粒:本质还是双链环状DNA 按照质粒本身的功能特性分类： 1. 克隆质粒：复制 2. 表达质粒：在克隆质粒的基础上含有启动子 3. 报导质粒：在表达质粒的基础上含有荧光、抗性基因等 4. shRNA质<br> 按照转基因后的质粒功能分类： 1. 过表达质粒：将目的基因插入报导质粒中， 2. 抑制表达...")
- 13:05, 8 July 2025 Hu yang talk contribs created page 细胞提取及分选 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==提取T细胞== #断颈处死小鼠，整体浸入含酒精的烧杯中灭菌3-5min，沿着腹部中线剪开取出脾脏，放入含PBS的6孔板中，转移至细胞房。 #换用新的6孔板或者50mlep管子(容纳筛网0.45），先加3 mL的2%FBS-PBS，放入筛网和脾脏，用针筒芯研磨至无明显固体 #转入灭菌EP管。离心2k rpm 3-5min，弃去上...")
- 11:50, 8 July 2025 Hu yang talk contribs created page File:细胞密度示意图.png
- 11:50, 8 July 2025 Hu yang talk contribs uploaded File:细胞密度示意图.png
- 03:39, 8 July 2025 Hu yang talk contribs created page 常用细胞房操作 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==更换培养基== #更换频率1次/d。 #弃去旧培养基： ##贴壁细胞（如癌细胞）：移液枪直接吸取培养基并弃去； ##悬浮细胞：收集原培养基及cell置于EP管中，离心，弃上清： ##半贴壁细胞：采用半换液，倾斜孔板或培养皿，移液枪吸取上层培养基弃去。 #加入新鲜培养基。培养基总量：10cm...")
- 12:21, 7 July 2025 Hu yang talk contribs uploaded a new version of File:琼脂糖凝胶电泳示意图.jpg
