User contributions for Hu yang
19 August 2025
- 09:0909:09, 19 August 2025 diff hist +18 N 2222 Hu yang moved page 2222 to 1111 current Tag: New redirect
- 09:0909:09, 19 August 2025 diff hist 0 m 1111 Hu yang moved page 2222 to 1111 current
- 09:0809:08, 19 August 2025 diff hist +4 N 1111 Created page with "Test"
- 09:0809:08, 19 August 2025 diff hist +10 HU:Project →PROTOCOLS
- 08:5908:59, 19 August 2025 diff hist +3 HU:Project No edit summary
- 03:3903:39, 19 August 2025 diff hist −14 HU:Protocols/琼脂糖凝胶电泳 →操作
- 03:3803:38, 19 August 2025 diff hist +782 HU:Protocols/琼脂糖凝胶电泳 →操作
- 03:2803:28, 19 August 2025 diff hist +11 HU:Protocols/琼脂糖凝胶电泳 →备注
- 03:2303:23, 19 August 2025 diff hist +122 HU:2025/Aug No edit summary
18 August 2025
- 09:3609:36, 18 August 2025 diff hist +441 N HU:Protocols/OligoTaC Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==OligoTaC制备== <ol> <li>单链DNA：100 uM in H2O <li>单价Oligotac制备 <ol> <li>制备一价双链DNA：分别取5 ul互补DNA单链，加入10 ul UP水 <li>37度摇床1.5 h <li>RT overnight </ol> <li>多价Oligotac制备 <ol>??? <li>取5ul上诉单价体系，加入多价单链DNA 5 ul <li>37度摇床1.5 h <li>琼脂糖凝胶验证"
- 09:2509:25, 18 August 2025 diff hist −6 HU:Project →PROTOCOLS
- 09:2409:24, 18 August 2025 diff hist +3 HU:Project No edit summary
- 09:2409:24, 18 August 2025 diff hist +10 HU:Project →PROTOCOLS
- 08:5308:53, 18 August 2025 diff hist 0 m HU:2025/Aug No edit summary
- 08:5308:53, 18 August 2025 diff hist +116 HU:2025/Aug No edit summary
- 08:4708:47, 18 August 2025 diff hist +65 HU:Protocols/常用细胞实验操作 →细胞保种
- 08:4408:44, 18 August 2025 diff hist +33 HU:Protocols/常用细胞实验操作 →细胞复苏
- 08:4008:40, 18 August 2025 diff hist +38 HU:Protocols/Ligation No edit summary
- 08:3708:37, 18 August 2025 diff hist +1 HU:Protocols/Ligation No edit summary
- 06:4906:49, 18 August 2025 diff hist +45 HU:Protocols/Ligation No edit summary
- 01:1701:17, 18 August 2025 diff hist +17 HU:Protocols/Ligation No edit summary
15 August 2025
- 11:1611:16, 15 August 2025 diff hist +59 HU:Protocols/常用细胞实验操作 No edit summary
- 11:1511:15, 15 August 2025 diff hist −18 HU:Protocols/常用细胞实验操作 No edit summary
- 11:1511:15, 15 August 2025 diff hist −32 HU:Protocols/常用细胞实验操作 No edit summary
- 11:1511:15, 15 August 2025 diff hist −8 HU:Protocols/常用细胞实验操作 No edit summary
- 11:1411:14, 15 August 2025 diff hist +106 HU:Protocols/常用细胞实验操作 →细胞保种
- 11:1211:12, 15 August 2025 diff hist +212 HU:Protocols/常用细胞实验操作 →更换培养基
- 11:0811:08, 15 August 2025 diff hist +4 HU:Protocols/常用细胞实验操作 →原理
- 11:0411:04, 15 August 2025 diff hist +12 HU:Protocols/蛋白提取及WB →WB
- 11:0311:03, 15 August 2025 diff hist +30 HU:Protocols/蛋白提取及WB →BCA测总蛋白
- 11:0111:01, 15 August 2025 diff hist 0 HU:Protocols/蛋白提取及WB →WB
- 11:0111:01, 15 August 2025 diff hist +6 HU:Protocols/蛋白提取及WB →WB
- 11:0011:00, 15 August 2025 diff hist +101 HU:Protocols/蛋白提取及WB →WB
- 09:0309:03, 15 August 2025 diff hist +87 HU:2025/Aug No edit summary
13 August 2025
- 08:2908:29, 13 August 2025 diff hist +23 HU:2025/Aug No edit summary
- 06:4606:46, 13 August 2025 diff hist −34 HU:Protocols/蛋白提取及WB →备注
- 06:4506:45, 13 August 2025 diff hist −37 HU:Protocols/蛋白提取及WB →备注
- 03:1703:17, 13 August 2025 diff hist +30 HU:Protocols/蛋白提取及WB →BCA测总蛋白
- 01:2501:25, 13 August 2025 diff hist +12 HU:Project No edit summary
12 August 2025
- 14:4714:47, 12 August 2025 diff hist +1,873 N HU:Protocols/Ligation Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <ol> <li><strong>Ligation Protocol with T4 DNA Ligase (M0202)</strong></br> <ol> <li>Set up the following reaction in a microcentrifuge tube on ice. ( Note that the table shows a ligation using a <strong>molar ratio</strong> of 1:3 vector to insert for the indicated DNA sizes.) Use NEBioCalculator to calculate molar ratios. <table class="wikitable" style="margin: 0 auto; bo..."
- 14:1214:12, 12 August 2025 diff hist −1 HU:Project →PROTOCOLS
- 13:5813:58, 12 August 2025 diff hist +76 HU:Protocols/细胞房注意事项 No edit summary
- 13:5713:57, 12 August 2025 diff hist −27 HU:Project →PROTOCOLS
- 13:5613:56, 12 August 2025 diff hist +18 N 常用细胞房操作 Hu yang moved page 常用细胞房操作 to HU:1 current Tag: New redirect
- 13:5613:56, 12 August 2025 diff hist 0 m HU:1 Hu yang moved page 常用细胞房操作 to HU:1 current
- 13:5513:55, 12 August 2025 diff hist −14,731 HU:1 Blanked the page Tag: Blanking
- 13:5313:53, 12 August 2025 diff hist +14,770 N HU:Protocols/常用细胞实验操作 Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==更换培养基== #更换频率1次/d。 #弃去旧培养基： ##贴壁细胞（如癌细胞）：移液枪直接吸取培养基并弃去； ##悬浮细胞：收集原培养基及cell置于EP管中，离心，弃上清： ##半贴壁细胞：采用半换液，倾斜孔板或培养皿，移液枪吸取上层培养基弃去。 #无需PBS清洗,直接加入新鲜培养基。..."
- 13:4513:45, 12 August 2025 diff hist +30 HU:Project →PROTOCOLS
- 13:4413:44, 12 August 2025 diff hist +19 HU:Protocols/流式 No edit summary
- 13:4213:42, 12 August 2025 diff hist +73 HU:2025/Aug No edit summary