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User contributions for Hu yang

For Hu yang talk block log uploads logs

A user with 845 edits. Account created on 30 April 2025.

13:4013:40, 12 August 2025 diff hist +4,478‎ N HU:Protocols/纳米粒 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <ol> <li> <strong>脂质体</strong> <ol> <li> <strong>胆固醇</strong> <p>胆固醇属于两亲物质，同时具有亲油和亲水两种基团，但是其亲油性较亲水性强，与磷脂相互作用形成脂质体时可嵌入到磷脂分子之间，调节膜结构及性质..."
13:2713:27, 12 August 2025 diff hist −10‎ HU:Project ‎ →‎PROTOCOLS
13:2613:26, 12 August 2025 diff hist +27‎ HU:Project ‎ →‎PROTOCOLS

14:2114:21, 11 August 2025 diff hist +157‎ N HU:2025/Aug ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *第三周 #cell铺板，脂质体制备，oligo体系合成"

07:3607:36, 26 July 2025 diff hist −2‎ HU:Protocols/流式 ‎No edit summary
06:5106:51, 26 July 2025 diff hist +3,932‎ N HU:Protocols/流式 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <big>'''一、操作'''</big> #CD8+ T cell单染PE-H(A) #人源性CD8 T MHCI-I染色：FBS溶液封闭+鼠抗人MHCI+山羊抗鼠荧光抗体 #进样前培养基更换为PBS，避免污染培养基。吸取染色后的cell，PBS稀释，Cell总量约为e6-e5，转移至流式管中500ul样本上样（每次进样仅需约30 ul） #阴性管：只有细胞，没有荧光抗..."
06:3006:30, 26 July 2025 diff hist −13‎ HU:Project ‎ →‎PROTOCOLS
03:3003:30, 26 July 2025 diff hist +2,489‎ N HU:Protocols/CCK8 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *<big>'''一、实验应用'''</big> 1. 细胞增殖测定 2. 细胞毒性测定 3. 细胞活性测定 (不加样本) 4. 药物筛选 5. 肿瘤药敏试验 6. 微生物对细胞毒性或增殖的测定 *<big>'''二、实验原理'''</big> CCK8全称Cell Counting Kit-8试剂，可用于简便而准确的细胞增殖和毒性分析。其基本原理为：该试剂中含有水..."
02:3602:36, 26 July 2025 diff hist +10‎ HU:Project ‎ →‎PROTOCOLS

13:4413:44, 22 July 2025 diff hist +4,640‎ N HU:Protocols/质粒 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> #质粒:本质还是双链环状DNA 按照质粒本身的功能特性分类： 1. 克隆质粒：复制 2. 表达质粒：在克隆质粒的基础上含有启动子 3. 报导质粒：在表达质粒的基础上含有荧光、抗性基因等 4. shRNA质<br> 按照转基因后的质粒功能分类： 1. 过表达质粒：将目的基因插入报导质粒中， 2. 抑制表达..."
13:3413:34, 22 July 2025 diff hist +214‎ HU:Project ‎ →‎PROTOCOLS
13:3313:33, 22 July 2025 diff hist +54‎ HU:2025/Jul ‎No edit summary current

10:0310:03, 19 July 2025 diff hist −4‎ HU:2025/Jul ‎No edit summary
10:0310:03, 19 July 2025 diff hist +108‎ HU:2025/Jul ‎No edit summary

03:1103:11, 14 July 2025 diff hist 0‎ HU:Project ‎ →‎学习记录
03:1103:11, 14 July 2025 diff hist +80‎ HU:2025/Jul ‎No edit summary

03:4403:44, 11 July 2025 diff hist +3‎ HU:Protocols/蛋白提取及WB ‎ →‎WB
03:1803:18, 11 July 2025 diff hist +529‎ HU:Protocols/蛋白提取及WB ‎ →‎原理
03:0703:07, 11 July 2025 diff hist +7‎ HU:Protocols/蛋白提取及WB ‎ →‎WB
03:0603:06, 11 July 2025 diff hist +15‎ HU:Protocols/蛋白提取及WB ‎ →‎WB
03:0603:06, 11 July 2025 diff hist +49‎ HU:Protocols/蛋白提取及WB ‎ →‎转膜

12:0512:05, 10 July 2025 diff hist +111‎ HU:Protocols/蛋白提取及WB ‎ →‎SDS-PAGE
08:1708:17, 10 July 2025 diff hist +242‎ HU:Protocols/蛋白提取及WB ‎ →‎BCA测总蛋白
07:1607:16, 10 July 2025 diff hist +348‎ HU:Protocols/蛋白提取及WB ‎ →‎备注
07:1307:13, 10 July 2025 diff hist −96‎ HU:Protocols/蛋白提取及WB ‎ →‎蛋白提取

06:3906:39, 9 July 2025 diff hist +55‎ HU:2025/Jul ‎No edit summary
03:3203:32, 9 July 2025 diff hist +57‎ HU:1 ‎ →‎12孔板CELL铺板（给药用）

13:1313:13, 8 July 2025 diff hist −18‎ HU:Project ‎No edit summary Tag: Manual revert
13:1213:12, 8 July 2025 diff hist −5,565‎ 细胞分选 ‎ Blanked the page current Tag: Blanking
13:1113:11, 8 July 2025 diff hist +38‎ HU:Protocols/细胞提取及分选 ‎ →‎流式测定 Tag: Manual revert
13:1113:11, 8 July 2025 diff hist −38‎ HU:Protocols/细胞提取及分选 ‎No edit summary Tag: Manual revert
13:1013:10, 8 July 2025 diff hist +38‎ HU:Protocols/细胞提取及分选 ‎No edit summary
13:0913:09, 8 July 2025 diff hist +18‎ HU:Project ‎ →‎PROTOCOLS
13:0513:05, 8 July 2025 diff hist +5,487‎ N HU:Protocols/细胞提取及分选 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==提取T细胞== #断颈处死小鼠，整体浸入含酒精的烧杯中灭菌3-5min，沿着腹部中线剪开取出脾脏，放入含PBS的6孔板中，转移至细胞房。 #换用新的6孔板或者50mlep管子(容纳筛网0.45），先加3 mL的2%FBS-PBS，放入筛网和脾脏，用针筒芯研磨至无明显固体 #转入灭菌EP管。离心2k rpm 3-5min，弃去上..."
12:1912:19, 8 July 2025 diff hist +45‎ HU:2025/Jul ‎No edit summary
12:1612:16, 8 July 2025 diff hist +8‎ HU:1 ‎ →‎原理
12:1612:16, 8 July 2025 diff hist +44‎ HU:1 ‎ →‎原理
12:1112:11, 8 July 2025 diff hist +638‎ HU:1 ‎ →‎原理
12:0712:07, 8 July 2025 diff hist +2,960‎ HU:1 ‎ →‎原理
11:5011:50, 8 July 2025 diff hist +70‎ HU:1 ‎ →‎细胞密度
11:5011:50, 8 July 2025 diff hist 0‎ N File:细胞密度示意图.png ‎No edit summary current
11:4711:47, 8 July 2025 diff hist 0‎ HU:Project ‎No edit summary
11:4611:46, 8 July 2025 diff hist +6‎ HU:Protocols/细胞房注意事项 ‎ →‎基本操作规范
11:4511:45, 8 July 2025 diff hist +98‎ HU:Protocols/细胞房注意事项 ‎ →‎生物安全柜使用流程
11:4311:43, 8 July 2025 diff hist +1‎ HU:Project ‎ →‎PROTOCOL
11:4311:43, 8 July 2025 diff hist +174‎ HU:1 ‎ →‎12孔板CELL铺板（给药用）
11:4211:42, 8 July 2025 diff hist +46‎ HU:1 ‎ →‎更换培养基
11:4011:40, 8 July 2025 diff hist +1,069‎ HU:1 ‎ →‎原理
10:4510:45, 8 July 2025 diff hist +61‎ HU:2025/Jul ‎No edit summary
10:3810:38, 8 July 2025 diff hist +207‎ HU:1 ‎No edit summary

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