User contributions for Hu yang

8 July 2025

• 10:3510:35, 8 July 2025 diff hist +359‎ HU:1 ‎ →‎细胞复苏
• 10:2110:21, 8 July 2025 diff hist +318‎ HU:Protocols/蛋白提取及WB ‎No edit summary
• 08:3208:32, 8 July 2025 diff hist +1,236‎ HU:1 ‎No edit summary
• 08:2408:24, 8 July 2025 diff hist +690‎ HU:1 ‎No edit summary
• 08:2108:21, 8 July 2025 diff hist −33‎ HU:1 ‎No edit summary
• 07:0407:04, 8 July 2025 diff hist +2,287‎ HU:1 ‎No edit summary
• 03:4303:43, 8 July 2025 diff hist −1‎ HU:1 ‎No edit summary
• 03:3903:39, 8 July 2025 diff hist +4,920‎ N HU:1 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==更换培养基== #更换频率1次/d。 #弃去旧培养基： ##贴壁细胞（如癌细胞）：移液枪直接吸取培养基并弃去； ##悬浮细胞：收集原培养基及cell置于EP管中，离心，弃上清： ##半贴壁细胞：采用半换液，倾斜孔板或培养皿，移液枪吸取上层培养基弃去。 #加入新鲜培养基。培养基总量：10cm..."
• 03:0303:03, 8 July 2025 diff hist +16‎ HU:2025/Jul ‎No edit summary
• 03:0203:02, 8 July 2025 diff hist −14‎ HU:Project ‎No edit summary
• 03:0103:01, 8 July 2025 diff hist +24‎ HU:Project ‎ →‎PROTOCOL
• 02:5902:59, 8 July 2025 diff hist +39‎ HU:Protocols/细胞房注意事项 ‎ →‎生物安全柜使用流程
• 02:5502:55, 8 July 2025 diff hist +766‎ HU:Protocols/蛋白提取及WB ‎ →‎BCA测总蛋白
• 02:4502:45, 8 July 2025 diff hist +23‎ HU:Protocols/蛋白提取及WB ‎No edit summary

7 July 2025

• 14:0014:00, 7 July 2025 diff hist +34‎ HU:2025/Jul ‎No edit summary
• 13:5913:59, 7 July 2025 diff hist +91‎ HU:2025/Jul ‎No edit summary
• 13:5213:52, 7 July 2025 diff hist +15‎ HU:Protocols/蛋白提取及WB ‎No edit summary
• 13:4813:48, 7 July 2025 diff hist +1,744‎ HU:Protocols/蛋白提取及WB ‎No edit summary
• 13:2413:24, 7 July 2025 diff hist +1,661‎ HU:Protocols/蛋白提取及WB ‎No edit summary
• 12:3312:33, 7 July 2025 diff hist +10‎ HU:Protocols/蛋白提取及WB ‎No edit summary
• 12:3112:31, 7 July 2025 diff hist +3,895‎ HU:Protocols/蛋白提取及WB ‎No edit summary
• 12:2112:21, 7 July 2025 diff hist 0‎ File:琼脂糖凝胶电泳示意图.jpg ‎ Hu yang uploaded a new version of File:琼脂糖凝胶电泳示意图.jpg current
• 12:2112:21, 7 July 2025 diff hist 0‎ File:WB实验.jpg ‎ Hu yang uploaded a new version of File:WB实验.jpg current
• 12:2012:20, 7 July 2025 diff hist 0‎ N File:SDS-PAGE制胶装置.jpg ‎No edit summary current
• 12:2012:20, 7 July 2025 diff hist 0‎ N File:SDS-PAGE电泳例图.jpg ‎No edit summary current
• 12:2012:20, 7 July 2025 diff hist 0‎ N File:SDS-PAGE凝胶比例.jpg ‎No edit summary current
• 12:1712:17, 7 July 2025 diff hist 0‎ N File:WB实验.jpg ‎No edit summary
• 12:1712:17, 7 July 2025 diff hist 0‎ N File:WB结果例图.png ‎No edit summary current
• 03:5103:51, 7 July 2025 diff hist +2,974‎ HU:Protocols/蛋白提取及WB ‎No edit summary
• 01:3901:39, 7 July 2025 diff hist −6‎ HU:Protocols/蛋白提取及WB ‎No edit summary
• 01:3701:37, 7 July 2025 diff hist +1,411‎ N HU:Protocols/蛋白提取及WB ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==目的== *药效（药效前应当先做毒性） ==蛋白提取== #移液枪转移12孔板中cell培养液到ep管中，底部留存部分液态培养基； #胰酶或者细胞刮板分离底部粘璧cell，移液枪转移至上述ep管中。流式不可采用刮板，会损伤cell；刮板用PBS清洗； #离心2k rpm 3min，弃去上清，PBS重悬，n合1，再次离心..."
• 01:0401:04, 7 July 2025 diff hist +23‎ HU:Project ‎ →‎常用实验方案

6 July 2025

• 12:0412:04, 6 July 2025 diff hist +2,856‎ N HU:Protocols/琼脂糖凝胶电泳 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==原理== # 分离：电泳（分子量，构象）+类似分子筛(小分子更快) # 成像：制胶溶液中的荧光分子和DNA结合 ==操作== # 参考实验视频：https://www.bilibili.com/video/BV1UR4y1x7ng/ # 制胶： ## 向锥形瓶中加入适量琼脂糖干粉和TAE电泳缓冲液50mL（Tris，乙酸，EDTA） ## 加热溶解，可超声or加磁力搅拌子..."
• 12:0212:02, 6 July 2025 diff hist 0‎ N File:凝胶模具示意图.jpg ‎No edit summary current
• 12:0212:02, 6 July 2025 diff hist 0‎ N File:琼脂糖凝胶电泳示意图.jpg ‎No edit summary
• 12:0212:02, 6 July 2025 diff hist 0‎ N File:琼脂糖凝胶结果图.png ‎No edit summary current
• 11:5611:56, 6 July 2025 diff hist +86‎ HU:2025/Jul ‎No edit summary

5 July 2025

• 04:0104:01, 5 July 2025 diff hist +1,777‎ N HU:Protocols/细胞房注意事项 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> === 基本操作规范 === #物品从传递窗进入 # 实验人员需穿戴：口罩、头套、手套、洁净服、换鞋 === 生物安全柜使用流程 === # 当日首次启用： ##开生物安全柜及整个cell房cell房紫外 ## 30mim后，关外间的紫外开关；开闸(黑色)，开空调通风10min后再进入。 # 开启步骤： ## 关紫外灯、开灯开..."
• 03:4703:47, 5 July 2025 diff hist +27‎ HU:Project ‎ →‎常用实验方案
• 02:5602:56, 5 July 2025 diff hist −1‎ 细胞分选 ‎ →‎备注
• 02:5502:55, 5 July 2025 diff hist +13‎ HU:Project ‎ →‎常用实验方案
• 02:5402:54, 5 July 2025 diff hist −80‎ HU:Project ‎ →‎常用实验方案
• 02:5302:53, 5 July 2025 diff hist +5,566‎ N 细胞分选 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> == 操作 == #取组织： ##断颈处死小鼠，整体浸入含酒精的烧杯中灭菌3-5min； ##沿着腹部中线剪开取出脾脏，放入含PBS的6孔板中，转移至细胞房。 #研磨： ## 换用新的6孔板或50ml EP管（用于容纳筛网0.45μm） ## 加3mL的2%FBS-PBS，放入筛网和脾脏，用针筒芯研磨至无明显固体，转入灭菌EP管，离..."
• 02:5102:51, 5 July 2025 diff hist 0‎ N File:CD8+T细胞分选试剂盒说明书.jpg ‎No edit summary current
• 02:5102:51, 5 July 2025 diff hist 0‎ N File:CFSE荧光强度随细胞分裂递减.jpg ‎No edit summary current
• 01:5301:53, 5 July 2025 diff hist +9‎ HU:Project ‎ →‎常用实验方案
• 01:5101:51, 5 July 2025 diff hist +10‎ HU:Project ‎No edit summary

4 July 2025

• 01:0101:01, 4 July 2025 diff hist +55‎ HU:2025/Jul ‎No edit summary

2 July 2025

• 12:4912:49, 2 July 2025 diff hist +9‎ HU:2025/Jul ‎No edit summary
• 09:1609:16, 2 July 2025 diff hist 0‎ File:CD8+ T细胞分选.pdf ‎ Hu yang uploaded a new version of File:CD8+ T细胞分选.pdf current