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Combined display of all available logs of yangwa. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 06:51, 26 July 2025 Hu yang talk contribs created page 流式 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> <big>'''一、操作'''</big> #CD8+ T cell单染PE-H(A) #人源性CD8 T MHCI-I染色：FBS溶液封闭+鼠抗人MHCI+山羊抗鼠荧光抗体 #进样前培养基更换为PBS，避免污染培养基。吸取染色后的cell，PBS稀释，Cell总量约为e6-e5，转移至流式管中500ul样本上样（每次进样仅需约30 ul） #阴性管：只有细胞，没有荧光抗...")
- 03:30, 26 July 2025 Hu yang talk contribs created page CCK8 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *<big>'''一、实验应用'''</big> 1. 细胞增殖测定 2. 细胞毒性测定 3. 细胞活性测定 (不加样本) 4. 药物筛选 5. 肿瘤药敏试验 6. 微生物对细胞毒性或增殖的测定 *<big>'''二、实验原理'''</big> CCK8全称Cell Counting Kit-8试剂，可用于简便而准确的细胞增殖和毒性分析。其基本原理为：该试剂中含有水...")
- 13:44, 22 July 2025 Hu yang talk contribs created page PCR (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> #质粒:本质还是双链环状DNA 按照质粒本身的功能特性分类： 1. 克隆质粒：复制 2. 表达质粒：在克隆质粒的基础上含有启动子 3. 报导质粒：在表达质粒的基础上含有荧光、抗性基因等 4. shRNA质<br> 按照转基因后的质粒功能分类： 1. 过表达质粒：将目的基因插入报导质粒中， 2. 抑制表达...")
- 13:05, 8 July 2025 Hu yang talk contribs created page 细胞提取及分选 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==提取T细胞== #断颈处死小鼠，整体浸入含酒精的烧杯中灭菌3-5min，沿着腹部中线剪开取出脾脏，放入含PBS的6孔板中，转移至细胞房。 #换用新的6孔板或者50mlep管子(容纳筛网0.45），先加3 mL的2%FBS-PBS，放入筛网和脾脏，用针筒芯研磨至无明显固体 #转入灭菌EP管。离心2k rpm 3-5min，弃去上...")
- 11:50, 8 July 2025 Hu yang talk contribs created page File:细胞密度示意图.png
- 11:50, 8 July 2025 Hu yang talk contribs uploaded File:细胞密度示意图.png
- 03:39, 8 July 2025 Hu yang talk contribs created page 常用细胞房操作 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==更换培养基== #更换频率1次/d。 #弃去旧培养基： ##贴壁细胞（如癌细胞）：移液枪直接吸取培养基并弃去； ##悬浮细胞：收集原培养基及cell置于EP管中，离心，弃上清： ##半贴壁细胞：采用半换液，倾斜孔板或培养皿，移液枪吸取上层培养基弃去。 #加入新鲜培养基。培养基总量：10cm...")
- 12:21, 7 July 2025 Hu yang talk contribs uploaded a new version of File:琼脂糖凝胶电泳示意图.jpg
- 12:21, 7 July 2025 Hu yang talk contribs uploaded a new version of File:WB实验.jpg
- 12:20, 7 July 2025 Hu yang talk contribs created page File:SDS-PAGE制胶装置.jpg
- 12:20, 7 July 2025 Hu yang talk contribs uploaded File:SDS-PAGE制胶装置.jpg
- 12:20, 7 July 2025 Hu yang talk contribs created page File:SDS-PAGE电泳例图.jpg
- 12:20, 7 July 2025 Hu yang talk contribs uploaded File:SDS-PAGE电泳例图.jpg
- 12:20, 7 July 2025 Hu yang talk contribs created page File:SDS-PAGE凝胶比例.jpg
- 12:20, 7 July 2025 Hu yang talk contribs uploaded File:SDS-PAGE凝胶比例.jpg
- 12:17, 7 July 2025 Hu yang talk contribs created page File:WB实验.jpg
- 12:17, 7 July 2025 Hu yang talk contribs uploaded File:WB实验.jpg
- 12:17, 7 July 2025 Hu yang talk contribs created page File:WB结果例图.png
- 12:17, 7 July 2025 Hu yang talk contribs uploaded File:WB结果例图.png
- 01:37, 7 July 2025 Hu yang talk contribs created page 蛋白提取及WB (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==目的== *药效（药效前应当先做毒性） ==蛋白提取== #移液枪转移12孔板中cell培养液到ep管中，底部留存部分液态培养基； #胰酶或者细胞刮板分离底部粘璧cell，移液枪转移至上述ep管中。流式不可采用刮板，会损伤cell；刮板用PBS清洗； #离心2k rpm 3min，弃去上清，PBS重悬，n合1，再次离心...")
- 12:04, 6 July 2025 Hu yang talk contribs created page 琼脂糖凝胶电泳 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==原理== # 分离：电泳（分子量，构象）+类似分子筛(小分子更快) # 成像：制胶溶液中的荧光分子和DNA结合 ==操作== # 参考实验视频：https://www.bilibili.com/video/BV1UR4y1x7ng/ # 制胶： ## 向锥形瓶中加入适量琼脂糖干粉和TAE电泳缓冲液50mL（Tris，乙酸，EDTA） ## 加热溶解，可超声or加磁力搅拌子...")
- 12:02, 6 July 2025 Hu yang talk contribs created page File:凝胶模具示意图.jpg
- 12:02, 6 July 2025 Hu yang talk contribs uploaded File:凝胶模具示意图.jpg
- 12:02, 6 July 2025 Hu yang talk contribs created page File:琼脂糖凝胶电泳示意图.jpg
- 12:02, 6 July 2025 Hu yang talk contribs uploaded File:琼脂糖凝胶电泳示意图.jpg
- 12:02, 6 July 2025 Hu yang talk contribs created page File:琼脂糖凝胶结果图.png
- 12:02, 6 July 2025 Hu yang talk contribs uploaded File:琼脂糖凝胶结果图.png
- 04:01, 5 July 2025 Hu yang talk contribs created page 细胞房注意事项 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> === 基本操作规范 === #物品从传递窗进入 # 实验人员需穿戴：口罩、头套、手套、洁净服、换鞋 === 生物安全柜使用流程 === # 当日首次启用： ##开生物安全柜及整个cell房cell房紫外 ## 30mim后，关外间的紫外开关；开闸(黑色)，开空调通风10min后再进入。 # 开启步骤： ## 关紫外灯、开灯开...")
- 02:53, 5 July 2025 Hu yang talk contribs created page 细胞分选 (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> == 操作 == #取组织： ##断颈处死小鼠，整体浸入含酒精的烧杯中灭菌3-5min； ##沿着腹部中线剪开取出脾脏，放入含PBS的6孔板中，转移至细胞房。 #研磨： ## 换用新的6孔板或50ml EP管（用于容纳筛网0.45μm） ## 加3mL的2%FBS-PBS，放入筛网和脾脏，用针筒芯研磨至无明显固体，转入灭菌EP管，离...")
- 02:51, 5 July 2025 Hu yang talk contribs created page File:CD8+T细胞分选试剂盒说明书.jpg
- 02:51, 5 July 2025 Hu yang talk contribs uploaded File:CD8+T细胞分选试剂盒说明书.jpg
- 02:51, 5 July 2025 Hu yang talk contribs created page File:CFSE荧光强度随细胞分裂递减.jpg
- 02:51, 5 July 2025 Hu yang talk contribs uploaded File:CFSE荧光强度随细胞分裂递减.jpg
- 09:16, 2 July 2025 Hu yang talk contribs uploaded a new version of File:CD8+ T细胞分选.pdf
- 09:08, 2 July 2025 Hu yang talk contribs uploaded a new version of File:转染及琼脂糖凝胶电泳.pdf
- 09:03, 2 July 2025 Hu yang talk contribs uploaded a new version of File:转染及琼脂糖凝胶电泳.pdf
- 09:00, 2 July 2025 Hu yang talk contribs uploaded a new version of File:转染及琼脂糖凝胶电泳.pdf
- 08:55, 2 July 2025 Hu yang talk contribs created page File:转染及琼脂糖凝胶电泳.pdf
- 08:55, 2 July 2025 Hu yang talk contribs uploaded File:转染及琼脂糖凝胶电泳.pdf
- 15:18, 1 July 2025 Hu yang talk contribs uploaded a new version of File:CD8+ T细胞分选.pdf
- 15:11, 1 July 2025 Hu yang talk contribs created page File:CD8+ T细胞分选.pdf
- 15:11, 1 July 2025 Hu yang talk contribs uploaded File:CD8+ T细胞分选.pdf
- 11:18, 1 July 2025 Hu yang talk contribs created page HU:2025/Jul (Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *01 小鼠脾脏中CD8+ T cell的分选（基于免疫磁微球试剂盒）、计数、铺板。 细胞流式仪用于试剂盒提取效率的考察，以及传达的考察。 </div>")
- 09:36, 1 July 2025 Hu yang talk contribs created page File:HY Head Photo.jpg
- 09:36, 1 July 2025 Hu yang talk contribs uploaded File:HY Head Photo.jpg
- 08:36, 23 May 2025 Hu yang talk contribs created page File talk:Scu.png (Created page with "T_T探索中，上传图片后没能删除，麻烦老师删除一下")
- 06:45, 23 May 2025 Hu yang talk contribs created page File:Scu.png
- 06:45, 23 May 2025 Hu yang talk contribs uploaded File:Scu.png
- 06:42, 23 May 2025 Hu yang talk contribs created page HU:2025/Jan (Created page with "=TEST= CONTENT")
- 04:01, 30 April 2025 User account Hu yang talk contribs was created automatically
