User contributions for Hu yang

01:3901:39, 7 July 2025 diff hist −6‎ HU:Protocols/蛋白提取及WB ‎No edit summary
01:3701:37, 7 July 2025 diff hist +1,411‎ N HU:Protocols/蛋白提取及WB ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==目的== *药效（药效前应当先做毒性） ==蛋白提取== #移液枪转移12孔板中cell培养液到ep管中，底部留存部分液态培养基； #胰酶或者细胞刮板分离底部粘璧cell，移液枪转移至上述ep管中。流式不可采用刮板，会损伤cell；刮板用PBS清洗； #离心2k rpm 3min，弃去上清，PBS重悬，n合1，再次离心..."
01:0401:04, 7 July 2025 diff hist +23‎ HU:Project ‎ →‎常用实验方案

12:0412:04, 6 July 2025 diff hist +2,856‎ N HU:Protocols/琼脂糖凝胶电泳 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==原理== # 分离：电泳（分子量，构象）+类似分子筛(小分子更快) # 成像：制胶溶液中的荧光分子和DNA结合 ==操作== # 参考实验视频：https://www.bilibili.com/video/BV1UR4y1x7ng/ # 制胶： ## 向锥形瓶中加入适量琼脂糖干粉和TAE电泳缓冲液50mL（Tris，乙酸，EDTA） ## 加热溶解，可超声or加磁力搅拌子..."
12:0212:02, 6 July 2025 diff hist 0‎ N File:凝胶模具示意图.jpg ‎No edit summary current
12:0212:02, 6 July 2025 diff hist 0‎ N File:琼脂糖凝胶电泳示意图.jpg ‎No edit summary
12:0212:02, 6 July 2025 diff hist 0‎ N File:琼脂糖凝胶结果图.png ‎No edit summary current
11:5611:56, 6 July 2025 diff hist +86‎ HU:2025/Jul ‎No edit summary

04:0104:01, 5 July 2025 diff hist +1,777‎ N HU:Protocols/细胞房注意事项 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> === 基本操作规范 === #物品从传递窗进入 # 实验人员需穿戴：口罩、头套、手套、洁净服、换鞋 === 生物安全柜使用流程 === # 当日首次启用： ##开生物安全柜及整个cell房cell房紫外 ## 30mim后，关外间的紫外开关；开闸(黑色)，开空调通风10min后再进入。 # 开启步骤： ## 关紫外灯、开灯开..."
03:4703:47, 5 July 2025 diff hist +27‎ HU:Project ‎ →‎常用实验方案
02:5602:56, 5 July 2025 diff hist −1‎ 细胞分选 ‎ →‎备注
02:5502:55, 5 July 2025 diff hist +13‎ HU:Project ‎ →‎常用实验方案
02:5402:54, 5 July 2025 diff hist −80‎ HU:Project ‎ →‎常用实验方案
02:5302:53, 5 July 2025 diff hist +5,566‎ N 细胞分选 ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> == 操作 == #取组织： ##断颈处死小鼠，整体浸入含酒精的烧杯中灭菌3-5min； ##沿着腹部中线剪开取出脾脏，放入含PBS的6孔板中，转移至细胞房。 #研磨： ## 换用新的6孔板或50ml EP管（用于容纳筛网0.45μm） ## 加3mL的2%FBS-PBS，放入筛网和脾脏，用针筒芯研磨至无明显固体，转入灭菌EP管，离..."
02:5102:51, 5 July 2025 diff hist 0‎ N File:CD8+T细胞分选试剂盒说明书.jpg ‎No edit summary current
02:5102:51, 5 July 2025 diff hist 0‎ N File:CFSE荧光强度随细胞分裂递减.jpg ‎No edit summary current
01:5301:53, 5 July 2025 diff hist +9‎ HU:Project ‎ →‎常用实验方案
01:5101:51, 5 July 2025 diff hist +10‎ HU:Project ‎No edit summary

01:0101:01, 4 July 2025 diff hist +55‎ HU:2025/Jul ‎No edit summary

12:4912:49, 2 July 2025 diff hist +9‎ HU:2025/Jul ‎No edit summary
09:1609:16, 2 July 2025 diff hist 0‎ File:CD8+ T细胞分选.pdf ‎ Hu yang uploaded a new version of File:CD8+ T细胞分选.pdf current
09:0809:08, 2 July 2025 diff hist 0‎ File:转染及琼脂糖凝胶电泳.pdf ‎ Hu yang uploaded a new version of File:转染及琼脂糖凝胶电泳.pdf current
09:0309:03, 2 July 2025 diff hist 0‎ File:转染及琼脂糖凝胶电泳.pdf ‎ Hu yang uploaded a new version of File:转染及琼脂糖凝胶电泳.pdf
09:0009:00, 2 July 2025 diff hist 0‎ File:转染及琼脂糖凝胶电泳.pdf ‎ Hu yang uploaded a new version of File:转染及琼脂糖凝胶电泳.pdf
08:5708:57, 2 July 2025 diff hist +50‎ HU:2025/Jul ‎No edit summary
08:5608:56, 2 July 2025 diff hist +79‎ HU:Project ‎ →‎常用实验方案
08:5508:55, 2 July 2025 diff hist 0‎ N File:转染及琼脂糖凝胶电泳.pdf ‎No edit summary

15:1815:18, 1 July 2025 diff hist 0‎ File:CD8+ T细胞分选.pdf ‎ Hu yang uploaded a new version of File:CD8+ T细胞分选.pdf
15:1415:14, 1 July 2025 diff hist +30‎ HU:Project ‎ →‎常用实验方案
15:1115:11, 1 July 2025 diff hist 0‎ N File:CD8+ T细胞分选.pdf ‎No edit summary
11:2911:29, 1 July 2025 diff hist −2‎ HU:Project ‎No edit summary
11:2911:29, 1 July 2025 diff hist +1‎ HU:Project ‎No edit summary
11:2811:28, 1 July 2025 diff hist −5‎ HU:Project ‎No edit summary
11:1811:18, 1 July 2025 diff hist +283‎ N HU:2025/Jul ‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *01 小鼠脾脏中CD8+ T cell的分选（基于免疫磁微球试剂盒）、计数、铺板。细胞流式仪用于试剂盒提取效率的考察，以及传达的考察。 </div>"
11:0511:05, 1 July 2025 diff hist +65‎ HU:Project ‎No edit summary
10:5710:57, 1 July 2025 diff hist −1‎ HU:Project ‎No edit summary
10:5310:53, 1 July 2025 diff hist −100‎ HU:Project ‎ →‎2025.07-: 学习纪要
10:5210:52, 1 July 2025 diff hist −1‎ HU:Project ‎ →‎常用实验方案
10:3810:38, 1 July 2025 diff hist +1,051‎ HU:Project ‎No edit summary
09:5509:55, 1 July 2025 diff hist +97‎ HU:Project ‎No edit summary
09:3609:36, 1 July 2025 diff hist 0‎ N File:HY Head Photo.jpg ‎No edit summary

08:3608:36, 23 May 2025 diff hist +69‎ N File talk:Scu.png ‎ Created page with "T_T探索中，上传图片后没能删除，麻烦老师删除一下" current
08:2608:26, 23 May 2025 diff hist −29‎ HU:2025/Jan ‎ →‎TEST current Tag: Manual revert
08:1908:19, 23 May 2025 diff hist −23‎ File:Scu.png ‎ Blanked the page current Tags: Blanking Manual revert
08:0508:05, 23 May 2025 diff hist +1‎ File:Scu.png ‎No edit summary
08:0508:05, 23 May 2025 diff hist +12‎ File:Scu.png ‎No edit summary
08:0108:01, 23 May 2025 diff hist +10‎ File:Scu.png ‎No edit summary
07:3507:35, 23 May 2025 diff hist +29‎ HU:2025/Jan ‎ →‎TEST
06:4506:45, 23 May 2025 diff hist 0‎ N File:Scu.png ‎No edit summary
06:4206:42, 23 May 2025 diff hist +14‎ N HU:2025/Jan ‎ Created page with "=TEST= CONTENT"

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