User contributions for Hu yang
7 July 2025
- 01:3901:39, 7 July 2025 diff hist −6 HU:Protocols/蛋白提取及WB No edit summary
- 01:3701:37, 7 July 2025 diff hist +1,411 N HU:Protocols/蛋白提取及WB Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==目的== *药效(药效前应当先做毒性) ==蛋白提取== #移液枪转移12孔板中cell培养液到ep管中,底部留存部分液态培养基; #胰酶或者细胞刮板分离底部粘璧cell,移液枪转移至上述ep管中。流式不可采用刮板,会损伤cell;刮板用PBS清洗; #离心2k rpm 3min,弃去上清,PBS重悬,n合1,再次离心..."
- 01:0401:04, 7 July 2025 diff hist +23 HU:Project →常用实验方案
6 July 2025
- 12:0412:04, 6 July 2025 diff hist +2,856 N HU:Protocols/琼脂糖凝胶电泳 Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> ==原理== # 分离:电泳(分子量,构象)+类似分子筛(小分子更快) # 成像:制胶溶液中的荧光分子和DNA结合 ==操作== # 参考实验视频:https://www.bilibili.com/video/BV1UR4y1x7ng/ # 制胶: ## 向锥形瓶中加入适量琼脂糖干粉和TAE电泳缓冲液50mL(Tris,乙酸,EDTA) ## 加热溶解,可超声or加磁力搅拌子..."
- 12:0212:02, 6 July 2025 diff hist 0 N File:凝胶模具示意图.jpg No edit summary current
- 12:0212:02, 6 July 2025 diff hist 0 N File:琼脂糖凝胶电泳示意图.jpg No edit summary
- 12:0212:02, 6 July 2025 diff hist 0 N File:琼脂糖凝胶结果图.png No edit summary current
- 11:5611:56, 6 July 2025 diff hist +86 HU:2025/Jul No edit summary
5 July 2025
- 04:0104:01, 5 July 2025 diff hist +1,777 N HU:Protocols/细胞房注意事项 Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> === 基本操作规范 === #物品从传递窗进入 # 实验人员需穿戴:口罩、头套、手套、洁净服、换鞋 === 生物安全柜使用流程 === # 当日首次启用: ##开生物安全柜及整个cell房cell房紫外 ## 30mim后,关外间的紫外开关;开闸(黑色),开空调通风10min后再进入。 # 开启步骤: ## 关紫外灯、开灯开..."
- 03:4703:47, 5 July 2025 diff hist +27 HU:Project →常用实验方案
- 02:5602:56, 5 July 2025 diff hist −1 细胞分选 →备注
- 02:5502:55, 5 July 2025 diff hist +13 HU:Project →常用实验方案
- 02:5402:54, 5 July 2025 diff hist −80 HU:Project →常用实验方案
- 02:5302:53, 5 July 2025 diff hist +5,566 N 细胞分选 Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> == 操作 == #取组织: ##断颈处死小鼠,整体浸入含酒精的烧杯中灭菌3-5min; ##沿着腹部中线剪开取出脾脏,放入含PBS的6孔板中,转移至细胞房。 #研磨: ## 换用新的6孔板或50ml EP管(用于容纳筛网0.45μm) ## 加3mL的2%FBS-PBS,放入筛网和脾脏,用针筒芯研磨至无明显固体,转入灭菌EP管,离..."
- 02:5102:51, 5 July 2025 diff hist 0 N File:CD8+T细胞分选试剂盒说明书.jpg No edit summary current
- 02:5102:51, 5 July 2025 diff hist 0 N File:CFSE荧光强度随细胞分裂递减.jpg No edit summary current
- 01:5301:53, 5 July 2025 diff hist +9 HU:Project →常用实验方案
- 01:5101:51, 5 July 2025 diff hist +10 HU:Project No edit summary
4 July 2025
- 01:0101:01, 4 July 2025 diff hist +55 HU:2025/Jul No edit summary
2 July 2025
- 12:4912:49, 2 July 2025 diff hist +9 HU:2025/Jul No edit summary
- 09:1609:16, 2 July 2025 diff hist 0 File:CD8+ T细胞分选.pdf Hu yang uploaded a new version of File:CD8+ T细胞分选.pdf current
- 09:0809:08, 2 July 2025 diff hist 0 File:转染及琼脂糖凝胶电泳.pdf Hu yang uploaded a new version of File:转染及琼脂糖凝胶电泳.pdf current
- 09:0309:03, 2 July 2025 diff hist 0 File:转染及琼脂糖凝胶电泳.pdf Hu yang uploaded a new version of File:转染及琼脂糖凝胶电泳.pdf
- 09:0009:00, 2 July 2025 diff hist 0 File:转染及琼脂糖凝胶电泳.pdf Hu yang uploaded a new version of File:转染及琼脂糖凝胶电泳.pdf
- 08:5708:57, 2 July 2025 diff hist +50 HU:2025/Jul No edit summary
- 08:5608:56, 2 July 2025 diff hist +79 HU:Project →常用实验方案
- 08:5508:55, 2 July 2025 diff hist 0 N File:转染及琼脂糖凝胶电泳.pdf No edit summary
1 July 2025
- 15:1815:18, 1 July 2025 diff hist 0 File:CD8+ T细胞分选.pdf Hu yang uploaded a new version of File:CD8+ T细胞分选.pdf
- 15:1415:14, 1 July 2025 diff hist +30 HU:Project →常用实验方案
- 15:1115:11, 1 July 2025 diff hist 0 N File:CD8+ T细胞分选.pdf No edit summary
- 11:2911:29, 1 July 2025 diff hist −2 HU:Project No edit summary
- 11:2911:29, 1 July 2025 diff hist +1 HU:Project No edit summary
- 11:2811:28, 1 July 2025 diff hist −5 HU:Project No edit summary
- 11:1811:18, 1 July 2025 diff hist +283 N HU:2025/Jul Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> *01 小鼠脾脏中CD8+ T cell的分选(基于免疫磁微球试剂盒)、计数、铺板。 细胞流式仪用于试剂盒提取效率的考察,以及传达的考察。 </div>"
- 11:0511:05, 1 July 2025 diff hist +65 HU:Project No edit summary
- 10:5710:57, 1 July 2025 diff hist −1 HU:Project No edit summary
- 10:5310:53, 1 July 2025 diff hist −100 HU:Project →2025.07-: 学习纪要
- 10:5210:52, 1 July 2025 diff hist −1 HU:Project →常用实验方案
- 10:3810:38, 1 July 2025 diff hist +1,051 HU:Project No edit summary
- 09:5509:55, 1 July 2025 diff hist +97 HU:Project No edit summary
- 09:3609:36, 1 July 2025 diff hist 0 N File:HY Head Photo.jpg No edit summary
23 May 2025
- 08:3608:36, 23 May 2025 diff hist +69 N File talk:Scu.png Created page with "T_T探索中,上传图片后没能删除,麻烦老师删除一下" current
- 08:2608:26, 23 May 2025 diff hist −29 HU:2025/Jan →TEST current Tag: Manual revert
- 08:1908:19, 23 May 2025 diff hist −23 File:Scu.png Blanked the page current Tags: Blanking Manual revert
- 08:0508:05, 23 May 2025 diff hist +1 File:Scu.png No edit summary
- 08:0508:05, 23 May 2025 diff hist +12 File:Scu.png No edit summary
- 08:0108:01, 23 May 2025 diff hist +10 File:Scu.png No edit summary
- 07:3507:35, 23 May 2025 diff hist +29 HU:2025/Jan →TEST
- 06:4506:45, 23 May 2025 diff hist 0 N File:Scu.png No edit summary
- 06:4206:42, 23 May 2025 diff hist +14 N HU:2025/Jan Created page with "=TEST= CONTENT"