Construction of DNA-Origami: Difference between revisions
| Line 17: | Line 17: | ||
! Volume（50 μL） | ! Volume（50 μL） | ||
|- | |- | ||
| Staples ( | | Staples (500 nM) | ||
| 25 μL | | 25 μL | ||
| 12.5 μL | | 12.5 μL | ||
|- | |- | ||
| P8064 ( | | P8064 (150 nM) | ||
| 10 μL | | 10 μL | ||
| 5 μL | | 5 μL | ||
Revision as of 13:36, 25 November 2025
Construction Material
Scaffold
- P8064 Powder and Stock Solution at 150 nM
Staples
- Stock solutions in three 96‑well plates at 100 uM
- Schematic Diagram
For detailed information, please refer to the shared "Origami" file on Feishu
- Stock solution in EP tube at 500 nM
Fabrication
| Material | Volume（100 μL） | Volume（50 μL） |
|---|---|---|
| Staples (500 nM) | 25 μL | 12.5 μL |
| P8064 (150 nM) | 10 μL | 5 μL |
| 10X PBS | 10 μL | 5 μL |
| H2O | 55 μL | 27.5 μL |