Construction of DNA-Origami: Difference between revisions
| Line 15: | Line 15: | ||
! 体积（100 μL） | ! 体积（100 μL） | ||
! 体积（50 μL） | ! 体积（50 μL） | ||
|- | |- | ||
| Staples (400 nM) | | Staples (400 nM) | ||
| 25 μL | | 25 μL | ||
| 12.5 μL | | 12.5 μL | ||
|- | |- | ||
| P8064 (200 nM) | | P8064 (200 nM) | ||
| 10 μL | | 10 μL | ||
| 5 μL | | 5 μL | ||
|- | |- | ||
| 10X PBS | | 10X PBS | ||
| 10 μL | | 10 μL | ||
| 5 μL | | 5 μL | ||
|- | |- | ||
| H2O | | H2O | ||
| 55 μL | | 55 μL | ||
| 27.5 μL | | 27.5 μL | ||
|} | |} | ||
Revision as of 13:31, 25 November 2025
Construction Material
Scaffold
- P8064 Powder and Stock Solution at 200 nM
Staples
- Stock solutions in three 96‑well plates at 100 nM
- Schematic Diagram
- For detailed information, please refer to the shared "Origami" file on Feishu
Fabrication
| 材料 | 体积（100 μL） | 体积（50 μL） |
|---|---|---|
| Staples (400 nM) | 25 μL | 12.5 μL |
| P8064 (200 nM) | 10 μL | 5 μL |
| 10X PBS | 10 μL | 5 μL |
| H2O | 55 μL | 27.5 μL |