Protocol 2

Protocol

pH

• Stock Solution: 1 mM

• Buffer: 0.5X TBE with 10 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5)

• Incubation: 37℃;C, 3 h

T-S1	T-S2	TFO	Buffer	pH
4 μL	4 μL	4 μL	388 μL	5.5 , 6.5 , 7.0 , 7.5 , 8.5

• Fluorometer