Protocol 2: Difference between revisions
(→pH) |
|||
| Line 8: | Line 8: | ||
*'''Incubation''': 37℃;C, 3 h | *'''Incubation''': 37℃;C, 3 h | ||
{| class="wikitable | {| class="wikitable"style="text-align:center; width:80%" | ||
! T-S1 | ! T-S1 | ||
! T-S2 | ! T-S2 | ||
Revision as of 07:23, 11 December 2025
Protocol
pH
- Stock Solution: 1 mM
- Buffer: 0.5X TBE with 11 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5)
- Incubation: 37℃;C, 3 h
| T-S1 | T-S2 | TFO | Buffer | pH |
|---|---|---|---|---|
| 4 μL | 4 μL | 4 μL | 388 μL | 5.5, 6.5, 7.0, 7.5, 8.5 |
- Fluorometer