Plasmids: Difference between revisions

Latest revision as of 18:12, 24 April 2025

@@ Line 1: / Line 1: @@
 ==CD19 CARs:==
+===PP7 and MS2:===
+*[[File:CRISPR-dCas9_and_sgRNA_scaffolds_enable_dual-colour_live_imaging_of_satellite_sequences_and_repeat-enriched_individual_loci.pdf]]
+*[[File:PP7 coat protein sequence Addgene.dna]]
+*[[File:MS2 coat protein sequences Addgene.dna]]
+===CD19 CARs:===
+*[[File: Gblocks order for MS2 coat protein included CD19 CAR.docx]]
+*[[File: PAS29 antiCD19 CAR Control corrected.dna]]
+*[[File: PAS29 antiCD19 CAR MS2 nearM corrected.dna.dna]]
+*[[File: PAS29 antiCD19 CAR MS2 nearCD3zeta corrected.dna]]
+*[[File: PAS29 antiCD19 CAR MS2 end corrected.dna]]
 ==RNA constructs:==
 *Linear RNA
 *Circular RNA
 *RNA origami
 ==TGF-beta sensors==
 *[[File:PAS48 14 TGF-b.dna|thumb]]
@@ Line 9: / Line 21: @@
 *[[File:PAS54 14 TGF-b TATA Box.dna|thumb]]
 *[[File:PAS55 16 TGF-b TATA Box.dna|thumb]]
+*[[File:PAS94 14TGF-b-(GAD, VP64-GFP) YBTATA.gb.zip|thumb]]
+*[[File:PAS94_14TGF-b-(GAD,_VP64-GFP)_LateADEp.gb.zip|thumb]]
 ===Results===
-*Produce virus from HEK, and transduce on HEK, with (+) or without adding TGF-beta in the medium:
+*[[File:TGFbeta sensor test on HEK cells 20250327.png|400px|TGF-beta sensor test on HEK 01]]
-*[[File:TGF_beta_sensor_tested_on_HEK_cells_yang_20250423.pdf|thumb]]