Protocol 2: Difference between revisions
(Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protocol == === pH ==== *'''Stock Solution''': 1 mM *'''Buffer''': 0.5X TBE with 11 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5) *'''Incubation''': 37℃;C, 3 h {| class="wikitable" ! T-S1 ! T-S2 ! TFO ! Buffer ! pH |- | 4 μL | 4 μL | 4 μL | 388 μL | 5.5, 6.5, 7.0, 7.5, 8.5 |} *Fluorometer") |
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<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> | <div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> | ||
== Protocol == | == Protocol == | ||
=== pH | === pH === | ||
*'''Stock Solution''': 1 mM | *'''Stock Solution''': 1 mM | ||
*'''Buffer''': 0.5X TBE with | *'''Buffer''': 0.5X TBE with 10 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5) | ||
*'''Incubation''': 37℃;C, 3 h | *'''Incubation''': 37℃;C, 3 h | ||
{| class="wikitable" | {| class="wikitable"style="text-align:center; width:80%" | ||
! T-S1 | ! T-S1 | ||
! T-S2 | ! T-S2 | ||
| Line 19: | Line 19: | ||
| 4 μL | | 4 μL | ||
| 388 μL | | 388 μL | ||
| 5.5, 6.5, 7.0, 7.5, 8.5 | | 5.5 , 6.5 , 7.0 , 7.5 , 8.5 | ||
|} | |} | ||
*Fluorometer | *Fluorometer | ||