Protocol 2: Difference between revisions
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(→pH) |
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*'''Stock Solution''': 1 mM | *'''Stock Solution''': 1 mM | ||
*'''Buffer''': 0.5X TBE with | *'''Buffer''': 0.5X TBE with 10 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5) | ||
*'''Incubation''': 37℃;C, 3 h | *'''Incubation''': 37℃;C, 3 h | ||
{| class="wikitable | {| class="wikitable"style="text-align:center; width:80%" | ||
! T-S1 | ! T-S1 | ||
! T-S2 | ! T-S2 | ||
| Line 19: | Line 19: | ||
| 4 μL | | 4 μL | ||
| 388 μL | | 388 μL | ||
| 5.5, 6.5, 7.0, 7.5, 8.5 | | 5.5 , 6.5 , 7.0 , 7.5 , 8.5 | ||
|} | |} | ||
*Fluorometer | *Fluorometer | ||