Protocol 2: Difference between revisions
(Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protocol == === pH ==== *'''Stock Solution''': 1 mM *'''Buffer''': 0.5X TBE with 11 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5) *'''Incubation''': 37℃;C, 3 h {| class="wikitable" ! T-S1 ! T-S2 ! TFO ! Buffer ! pH |- | 4 μL | 4 μL | 4 μL | 388 μL | 5.5, 6.5, 7.0, 7.5, 8.5 |} *Fluorometer") |
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Revision as of 07:21, 11 December 2025
Protocol
pH =
- Stock Solution: 1 mM
- Buffer: 0.5X TBE with 11 mM MgCl2 (pH: 5.5, 6.5, 7.0, 7.5, 8.5)
- Incubation: 37℃;C, 3 h
| T-S1 | T-S2 | TFO | Buffer | pH |
|---|---|---|---|---|
| 4 μL | 4 μL | 4 μL | 388 μL | 5.5, 6.5, 7.0, 7.5, 8.5 |
- Fluorometer