Construction of DNA-Origami: Difference between revisions

Latest revision as of 14:00, 25 November 2025

Construction Material[edit]

Scaffold[edit]

P8064 Powder and Stock Solution at 150 nM

Staples[edit]

Stock solutions 1 (Hexagonal-prism DNA origami) in three 96‑well plates at 100 uM

Schematic Diagram

For detailed information, please refer to the shared "Origami" file on Feishu

Stock solutions 2 (Unmodified hexagonal‑prism DNA origami) in EP tube at 500 nM

Fabrication[edit]

1. Folfing

Material	Volume（100 μL）	Volume（50 μL）
Staples (500 nM)	25 μL	12.5 μL
P8064 (150 nM)	10 μL	5 μL
10X PBS	10 μL	5 μL
H2O	55 μL	27.5 μL

Program folding in PCR thermocycler : The mixed sample was put on a thermal ramp starting with a rapid heat denaturation at 80 °C for 5 min followed by cooling from 80 °C to 60 °C over 20 min, then slow cooling from 60 °C to 24 °C over 14 h.

2. Purifying

Six times ultrafiltration using a 100 kDa molecular-weight-cutoff filter(MWCO)
2% agarose gel electrophoresis running at 90 V for 2 h. Louding(Marker, Scaffold, Samples without ultrafiltration, Samples with ultrafiltration, Marker)
Image development

3. TME

Observation by negative‑stain transmission electron microscopy

@@ Line 2: / Line 2: @@
 == Construction Material ==
 === Scaffold ===
-#'''P8064''' '''Powder and Stock Solution at 200 nM'''
+#'''P8064''' '''Powder and Stock Solution at 150 nM'''
 === Staples ===
-#'''Stock solutions in three 96‑well plates at 100 nM'''
+*'''Stock solutions 1 (Hexagonal-prism DNA origami) in three 96‑well plates at 100 uM'''
-*'''Schematic Diagram'''
+#'''Schematic Diagram'''
-[[File:Staples.png|thumb|center|800px|Schematic Diagram]]
+[[File:Staples.png|thumb|center|400px|Schematic Diagram]]
+For detailed information, please refer to the shared "Origami" file on Feishu
+*'''Stock solutions 2 (Unmodified hexagonal‑prism DNA origami) in EP tube at 500 nM'''
+== Fabrication ==
+*'''''1. Folfing '''''
+{| class="wikitable"
+! Material
+! Volume（100 μL）
+! Volume（50 μL）
+|-
+| Staples (500 nM)
+| 25 μL
+| 12.5 μL
+|-
+| P8064 (150 nM)
+| 10 μL
+| 5 μL
+|-
+| 10X PBS
+| 10 μL
+| 5 μL
+|-
+| H2O
+| 55 μL
+| 27.5 μL
+|}
+#'''Program folding in PCR thermocycler : The mixed sample was put on a thermal ramp starting with a rapid heat denaturation at 80 °C for 5 min followed by cooling from 80 °C to 60 °C over 20 min, then slow cooling from 60 °C to 24 °C over 14 h.'''
+*'''''2. Purifying'''''
+#''' Six times ultrafiltration using a 100 kDa molecular-weight-cutoff filter(MWCO)'''
+#''' 2% agarose gel electrophoresis running at 90 V for 2 h.''' Louding(Marker, Scaffold, Samples without ultrafiltration, Samples with ultrafiltration, Marker)
+#''' Image development'''
+*
+*'''''3. TME '''''
+*
+#'''Observation by negative‑stain transmission electron microscopy'''