XIE/2:2026/Jan: Revision history

28 January 2026

• curprev 12:5512:55, 28 January 2026‎ Xie guangneng talk contribs‎ 4,217 bytes 0‎ →‎1.28 undo
• curprev 12:5512:55, 28 January 2026‎ Xie guangneng talk contribs‎ 4,217 bytes +3‎ →‎1.28 undo
• curprev 12:5512:55, 28 January 2026‎ Xie guangneng talk contribs‎ 4,214 bytes +3‎ →‎1.28 undo
• curprev 12:5412:54, 28 January 2026‎ Xie guangneng talk contribs‎ 4,211 bytes +221‎ →‎平均偶联数量测量 undo
• curprev 12:4612:46, 28 January 2026‎ Xie guangneng talk contribs‎ 3,990 bytes +550‎ No edit summary undo

26 January 2026

• curprev 08:2808:28, 26 January 2026‎ Xie guangneng talk contribs‎ 3,440 bytes +1‎ →‎1.19 undo
• curprev 08:2808:28, 26 January 2026‎ Xie guangneng talk contribs‎ 3,439 bytes +47‎ No edit summary undo
• curprev 08:0808:08, 26 January 2026‎ Xie guangneng talk contribs‎ 3,392 bytes +74‎ →‎Native-page undo
• curprev 08:0808:08, 26 January 2026‎ Xie guangneng talk contribs‎ 3,318 bytes +73‎ →‎Native-page undo
• curprev 08:0708:07, 26 January 2026‎ Xie guangneng talk contribs‎ 3,245 bytes +65‎ →‎1.21 undo
• curprev 08:0408:04, 26 January 2026‎ Xie guangneng talk contribs‎ 3,180 bytes +21‎ →‎Native-page undo
• curprev 08:0408:04, 26 January 2026‎ Xie guangneng talk contribs‎ 3,159 bytes +107‎ →‎1.20 undo

22 January 2026

• curprev 05:3705:37, 22 January 2026‎ Xie guangneng talk contribs‎ 3,052 bytes +36‎ →‎1.13 undo
• curprev 04:5504:55, 22 January 2026‎ Xie guangneng talk contribs‎ 3,016 bytes +195‎ →‎1.20 undo
• curprev 04:5004:50, 22 January 2026‎ Xie guangneng talk contribs‎ 2,821 bytes +905‎ →‎1.19 undo
• curprev 04:3404:34, 22 January 2026‎ Xie guangneng talk contribs‎ 1,916 bytes +149‎ →‎1.19 undo
• curprev 04:3104:31, 22 January 2026‎ Xie guangneng talk contribs‎ 1,767 bytes +601‎ No edit summary undo

18 January 2026

• curprev 13:4713:47, 18 January 2026‎ Xie guangneng talk contribs‎ 1,166 bytes +41‎ No edit summary undo

13 January 2026

• curprev 14:4514:45, 13 January 2026‎ Xie guangneng talk contribs‎ 1,125 bytes +2‎ No edit summary undo
• curprev 14:4114:41, 13 January 2026‎ Xie guangneng talk contribs‎ 1,123 bytes +37‎ No edit summary undo
• curprev 14:3814:38, 13 January 2026‎ Xie guangneng talk contribs‎ 1,086 bytes −1‎ →‎偶联= undo
• curprev 14:3514:35, 13 January 2026‎ Xie guangneng talk contribs‎ 1,087 bytes +114‎ No edit summary undo
• curprev 14:3514:35, 13 January 2026‎ Xie guangneng talk contribs‎ 973 bytes +973‎ Created page with "==1.13== ===抗体还原=== *取2 ul(1mM) TCEP,用PBS(7.4)稀释至 20 ul(100uM),取5 ul稀释后的TCEP，加入85 ul PBS(7.40)，涡旋混匀---TCEP溶液pH过低提前中和 *取2 ul(50uM)抗体，用PBS（pH 7.4）稀释至10 ul（10uM），将TECP的PBS缓冲液加入其中，37℃孵育2h. *取1 ul(50uM)抗体，用PBS（pH7.4）稀释至8 ul，作为 C 组 ==偶联=== *将孵育好的溶液用1XPBS(6.5)洗涤4次（10000g 3.5min），均分样品（A.B）----buffe..."