细胞分选: Revision history

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5 July 2025

  • curprev 02:5602:56, 5 July 2025Hu yang talk contribs 5,565 bytes −1 →‎备注 undo
  • curprev 02:5302:53, 5 July 2025Hu yang talk contribs 5,566 bytes +5,566 Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important;"> == 操作 == #取组织: ##断颈处死小鼠,整体浸入含酒精的烧杯中灭菌3-5min; ##沿着腹部中线剪开取出脾脏,放入含PBS的6孔板中,转移至细胞房。 #研磨: ## 换用新的6孔板或50ml EP管(用于容纳筛网0.45μm) ## 加3mL的2%FBS-PBS,放入筛网和脾脏,用针筒芯研磨至无明显固体,转入灭菌EP管,离..."