Protocol: Revision history

3 November 2025

• curprev 11:3911:39, 3 November 2025‎ Xie guangneng talk contribs‎ 3,392 bytes +1‎ →‎一、酶核酸杂交体的制备 undo
• curprev 11:3811:38, 3 November 2025‎ Xie guangneng talk contribs‎ 3,391 bytes −1‎ →‎Protocol (版本 1.0) undo
• curprev 11:3811:38, 3 November 2025‎ Xie guangneng talk contribs‎ 3,392 bytes +6‎ →‎Protocol (版本 1.0) undo
• curprev 11:3711:37, 3 November 2025‎ Xie guangneng talk contribs‎ 3,386 bytes −4‎ →‎Protocol (版本 1.0) undo
• curprev 11:3711:37, 3 November 2025‎ Xie guangneng talk contribs‎ 3,390 bytes +5‎ →‎一、酶核酸杂交体的制备 undo
• curprev 11:3711:37, 3 November 2025‎ Xie guangneng talk contribs‎ 3,385 bytes +6‎ →‎一、酶核酸杂交体的制备 undo
• curprev 11:3611:36, 3 November 2025‎ Xie guangneng talk contribs‎ 3,379 bytes −30‎ →‎Protocol (版本 1.0) undo
• curprev 11:3611:36, 3 November 2025‎ Xie guangneng talk contribs‎ 3,409 bytes 0‎ →‎Protocol (版本 1.0) undo
• curprev 11:3511:35, 3 November 2025‎ Xie guangneng talk contribs‎ 3,409 bytes −2‎ →‎Protocol (版本 1.0) undo
• curprev 11:3511:35, 3 November 2025‎ Xie guangneng talk contribs‎ 3,411 bytes +146‎ →‎Protocol (版本 1.0) undo
• curprev 11:3011:30, 3 November 2025‎ Xie guangneng talk contribs‎ 3,265 bytes 0‎ →‎Protocol (版本 1.0) undo
• curprev 11:3011:30, 3 November 2025‎ Xie guangneng talk contribs‎ 3,265 bytes +48‎ →‎Protocol (版本 1.0) undo
• curprev 08:3808:38, 3 November 2025‎ Xie guangneng talk contribs‎ 3,217 bytes −2‎ →‎5. 向 GDH 样品液中加入 DBCO，并在室温下孵育 4 h undo
• curprev 08:3808:38, 3 November 2025‎ Xie guangneng talk contribs‎ 3,219 bytes −1‎ →‎4. 稀释 DBCO（样品液） undo

1 November 2025

• curprev 05:4105:41, 1 November 2025‎ Xie guangneng talk contribs‎ 3,220 bytes +18‎ →‎8. 凝胶电泳 undo
• curprev 05:4005:40, 1 November 2025‎ Xie guangneng talk contribs‎ 3,202 bytes −1‎ →‎7. Oligo undo
• curprev 05:2405:24, 1 November 2025‎ Xie guangneng talk contribs‎ 3,203 bytes −3‎ →‎7. 加入 Oligo，并在 37 ℃ 下孵育 4 h undo
• curprev 05:2105:21, 1 November 2025‎ Xie guangneng talk contribs‎ 3,206 bytes 0‎ →‎7. Oligo undo
• curprev 05:2005:20, 1 November 2025‎ Xie guangneng talk contribs‎ 3,206 bytes −5‎ →‎7. Oligo undo
• curprev 05:1905:19, 1 November 2025‎ Xie guangneng talk contribs‎ 3,211 bytes +303‎ →‎6. 使用 10 kDa 超滤管超滤去除游离 DBCO undo
• curprev 05:1105:11, 1 November 2025‎ Xie guangneng talk contribs‎ 2,908 bytes −1‎ →‎7. 加入 Oligo，并在 37 ℃ 下孵育 4 h undo
• curprev 04:5004:50, 1 November 2025‎ Xie guangneng talk contribs‎ 2,909 bytes +6‎ →‎3. 溶解 DBCO-PEG4-Bis-Sulfone 于 DMF(N,N-二甲基甲酰胺) 中（100 mM） undo
• curprev 04:5004:50, 1 November 2025‎ Xie guangneng talk contribs‎ 2,903 bytes 0‎ →‎3. 溶解 DBCO-PEG4-Bis-Sulfone 于 DMF(N,N-二甲基甲酰胺) 中（100 mM） undo
• curprev 04:4904:49, 1 November 2025‎ Xie guangneng talk contribs‎ 2,903 bytes −1‎ →‎3. 溶解 DBCO-PEG4-Bis-Sulfone 于 DMF(N,N-二甲基甲酰胺) 中（100 mM） undo
• curprev 04:4904:49, 1 November 2025‎ Xie guangneng talk contribs‎ 2,904 bytes +42‎ →‎3. 溶解 DBCO-PEG4-Bis-Sulfone 于 DMF(N,N-二甲基甲酰胺) 中（100 mM） undo
• curprev 04:4404:44, 1 November 2025‎ Xie guangneng talk contribs‎ 2,862 bytes 0‎ →‎1. GDH 制备 undo

31 October 2025

• curprev 10:2910:29, 31 October 2025‎ Xie guangneng talk contribs‎ 2,862 bytes −4‎ →‎1. GDH 制备 undo
• curprev 08:2208:22, 31 October 2025‎ Xie guangneng talk contribs‎ 2,866 bytes +2,866‎ Created page with "<div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;"> == Protein(RuCHMO/GDH) Conjugation to DNA Origami == === Methods === === Conjugation === *''' Proteins containing 6x Histidine at the C terminus reacted, with the chemical DBCO-PEG4-Bis-sulfone(DPBS) for 4 h at room temperature. The bis-sulfone group reacts with the Histidines at the C terminus of the protein, and then an azide modified oligonucleotide is con..."