Editing Construction of DNA-Origami

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== Construction Material ==
=== Scaffold ===
#'''P8064''' '''Powder and Stock Solution at 150 nM'''

=== Staples ===
*'''Stock solutions 1 (Hexagonal-prism DNA origami) in three 96‑well plates at 100 uM'''
#'''Schematic Diagram'''
[[File:Staples.png|thumb|center|400px|Schematic Diagram]]
For detailed information, please refer to the shared "Origami" file on Feishu
*'''Stock solutions 2 (Unmodified hexagonal‑prism DNA origami) in EP tube at 500 nM'''

== Fabrication ==
*'''''1. Folfing '''''
{| class="wikitable"
! Material
! Volume（100 μL）
! Volume（50 μL）
|-
| Staples (500 nM)
| 25 μL
| 12.5 μL
|-
| P8064 (150 nM)
| 10 μL
| 5 μL
|-
| 10X PBS
| 10 μL
| 5 μL
|-
| H2O
| 55 μL
| 27.5 μL
|}
#'''Program folding in PCR thermocycler : The mixed sample was put on a thermal ramp starting with a rapid heat denaturation at 80 °C for 5 min followed by cooling from 80 °C to 60 °C over 20 min, then slow cooling from 60 °C to 24 °C over 14 h.'''

*'''''2. Purifying'''''
#''' Six times ultrafiltration using a 100 kDa molecular-weight-cutoff filter(MWCO)'''
#''' 2% agarose gel electrophoresis running at 90 V for 2 h.''' Louding(Marker, Scaffold, Samples without ultrafiltration, Samples with ultrafiltration, Marker)
#''' Image development'''
*
*'''''3. TME '''''
*
#'''Observation by negative‑stain transmission electron microscopy'''