Editing XIE/Enzyme:2025/Dec (section)

== 12.13 ==
*'''<big><span style="color:red;">S2 conjugated G5</span></big>'''
==='''Incubation'''===
#Prepare two aliquots of G5 stock, labeled A and B.
#Add 1 µL DBCO to each of A and B and incubate for 4 h.
#For A: remove 16 µL as sample M1; add 10 µL S2 to the remaining A (named O1) and incubate for 4 h.
#For B: dilute the sample to 500 µL, transfer to a 10 kDa centrifugal ultrafiltration tube and centrifuge at 10,000 g for 30 min; concentrate to 100 µL, remove 16 µL as sample M2; add 10 µL S2 to the remaining B (named O2) and incubate for 4 h