Editing XIE/Enzyme:2025/Dec (section)

== 12.10 ==
* <big>'''Agarose Gel Electrophoresis '''</big>
*'''Gel Preparation'''
#Recipe: 2.4 g agarose in 120 mL 0.5× TBE 
#Heat in a microwave to dissolve, cool slightly, adjust volume with 0.5× TBE to 120 ul, then add 1.2 mL of 1 M MgCl2 and 12 µL GoldView DNA stain.
#Insert comb and waite for the gel to cool and solidify.
*'''Sample preparation'''
#Origami (15 nM): sample:loading buffer = 5:1.
#Scaffold: take 1 µL scaffold, dilute to 10 µL (final concentration 15 nM); scaffold:loading buffer = 5:1.
*'''Electrophoresis'''
#Run at 90 V for 120 min in 0.5× TBE with 10 mM MgCl2
#'''Marker、Scaffold（1X）、Origami（10.08-未洗）、Origami（10.08—清洗）、Origami（10.09-未洗）、Origami（10.09-清洗）、Scaffold（10X）、Marker'''
[[FIle:Origami(NO)_25_12_10.png|thumb|center|600px|Origami(NO)]]