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===琼脂糖凝胶电泳(1.23)=== #2.4g琼脂糖加入到120 ml(0.5X TBE)中,微波炉加入至完全溶解;用 0.5X TBE 补足,再加入 1.2ml 1M MgCl2 和 12 ul Goldview;倒入模具,插上梳子。 #取Scaffold、Origami(50nM)、Origami(100nM)、Origami(150nM)各 10 ul, 各加入 Loudingbuffer 2 ul,涡旋混匀。 #Scaffold、Origami(50nM)、Origami(100nM)、Origami(150nM)依次上样,90V 3h(电泳液:0.5X TBE + 10mM MgCl2) [[File:T_Origami_2026-01-23.jpg|thumb|center|400px]] <span style="color:red;">结论:150 nM 浓度太低</span> <div style="color: black !important; font-family: 'Times New Roman', SimSun, serif !important, line-height:1.5;">
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