Editing XIE/3:2026/Jan (section)

==1.22==
*<big><span style="color:red;">'''Origami folding'''</span></big>
===折叠三种浓度的Origami（50nM,100nM,150nM）===
{| class="wikitable"
|+ 50 nM
! Samples
! Volume
|-
| Scaffolld (1uM)
| 5 ul
|-
| Staples (10uM)
| 2.5 ul X 9
|-
| PBS (10X)
| 10 ul
|-
| Water
| 62.5 ul
|}
{| class="wikitable"
|+ 100 nM
! Samples
! Volume
|-
| Scaffolld (1uM)
| 10 ul
|-
| Staples (10uM)
| 5 ul X 9
|-
| PBS (10X)
| 10 ul
|-
| Water
| 35 ul
|}
{| class="wikitable"
|+ 150 nM
! Samples
! Volume
|-
| Scaffolld (1uM)
| 15 ul
|-
| Staples (10uM)
| 7.5 ul X 9
|-
| PBS (10X)
| 10 ul
|-
| Water
| 7.5 ul
|}
*
*Program folding in PCR thermocycler : The mixed sample was put on a thermal ramp starting with '''a rapid heat denaturation at 80 °C for 5 min followed by cooling from 80 °C to 60 °C over 20 min, then slow cooling from 60 °C to 24 °C over 14 h'''.

*Purification :The incubated samples were mixed with 400 μL of phosphate‑buffered saline (PBS) and transferred into 50 kDa molecular‑weight‑cut‑off (MWCO) centrifugal ultrafiltration units. Samples were centrifuged at 10,000 × g for 3 min, the retentate was brought back to volume with PBS, and the centrifugal ultrafiltration step was repeated; this wash/centrifugation cycle was performed a total of three times
===琼脂糖凝胶电泳(1.23)===
#2.4g琼脂糖加入到120 ml（0.5X TBE）中，微波炉加入至完全溶解；用 0.5X TBE 补足，再加入 1.2ml 1M MgCl2 和 12 ul Goldview；倒入模具，插上梳子。
#取Scaffold、Origami（50nM）、Origami（100nM）、Origami（150nM）各 10 ul， 各加入 Loudingbuffer 2 ul，涡旋混匀。
#Scaffold、Origami（50nM）、Origami（100nM）、Origami（150nM）依次上样，90V 3h（电泳液：0.5X TBE + 10mM MgCl2）
[[File:T_Origami_2026-01-23.jpg|thumb|center|400px]]
<span style="color:red;">结论：150 nM 浓度太低</span>
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